Abstract
A systematic approach to process optimization for production of rifamycin B was applied to a strain of Amycolatopsis mediterranei . Examination of the growth revealed 6 different morphologically distinct colonies on Bennett's agar medium. Rifamycin B production in shake flasks by the six different colony types ranged between 0.5 and 1.2 g/l. There was a clear correlation between the colony morphology and rifamycin B productivity. The highest yield of rifamycin B (1.03-1.2 g/l) was obtained by using the orange-red colored colonies, rosette shaped, devoid of hollow center and 2-3 mm in diameter. Variability in colony morphology, however, remained and the appropriate colonies had to be picked up for preparing the inoculum of each experiment. Addition of yeast extract to the fermentation medium at different times increased rifamycin B production. The highest antibiotic production was obtained upon the addition of 0.1% yeast extract after 2 days of incubation, where the yield increased from 1.15 to 1.95 g/l (70%). The use of 1.8% KNO 3 in the fermentation medium, instead of 0.96% (NH 4 ) 2 SO 4 , markedly increased rifamycin B production from 1.15 to 2.92 g/l (154%). It was also observed, upon microscopical examination, that KNO 3 decreased branching and fragmentation of the mycelia in the fermentation medium. Keywords: Rifamycin B; fermentation; biotechnology; Amycolatopsis mediterranei; strain selection. African Journal of Biotechnology Vol.3(5) 2004: 266-272
Highlights
IntroductionTuberculosis remains a leading cause of death worldwide
Among infectious diseases, tuberculosis remains a leading cause of death worldwide
This study aimed at selection of the most active antibiotic producing colony from A. mediterranei–RCP 1001 (N1) strain, which suffered generally from low and inconsistent productivity of around 500 mg/l of rifamycin B
Summary
Tuberculosis remains a leading cause of death worldwide. It is thought that as many as 2 billion people have been exposed to the tuberculosis bacillus and are at risk of developing active disease. This problem is further compounded by a dramatic increase in multidrug-resistant strains of Mycobacterium tuberculosis. Apart from its application against pathogens of tuberculosis and leprosy, it has been found to be effective against several pathogens including Mycobacterium avium and penicillin-resistant pneumococci
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
