Abstract
The present study was designed to investigate the hepatoprotective and antioxidant properties of corosolic acid (CRA) on carbon tetrachloride (CCl4)-induced liver damage in rats. Liver damage was induced by giving a single oral dose of CCl4 (1:1 in liquid paraffin) at 1.25 ml/kg body weight (BW). Rats were pretreated with CRA dose of 10, 20 and 40 mg/kg BW (once daily for 7 days before CCl4 intoxication). Pretreatment with CRA showed significant hepatoprotection by reducing the aspartate transaminase (AST), alanine transaminase (ALT), and alkaline phosphatase (ALP) enzymatic activities which had been raised by CCl4administration. The levels of lipid peroxidation markers such as thiobarbituric acid reactive substances (TBARS) and lipid hydroperoxides (LOOH) were significantly increased by CCl4administration and pretreatment with CRA; the levels of lipid peroxidative markers were reduced. The activities of enzymic antioxidants (superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx)) and the levels of non enzymic antioxidants (Vitamins C, Vitamins E and reduced glutathione (GSH)) were decreased by CCl4 administration and those pretreated with CRA above enzymic and non enzymic antioxidants were increased. The present study concluded that CRA possesses hepatoprotective and antioxidant properties against CCl4-induced hepatotoxicity in rats. Key words: Hepatotoxicity, carbon tetrachloride (CCl4), corosolic acid, lipids peroxidations, antioxidant.
Highlights
The liver is a vital organ present in vertebrates and some other animals
The present study demonstrates the hepatoprotective, curative and antioxidant effects of corosolic acid (CRA) against CCl4induced liver injury in rats
Liver injury induced by CCl4 is a common model for screening the hepatoprotective activity of drugs, because this chemical is a potent hepatotoxin and a single exposure can rapidly lead to severe hepatic necrosis and steatosis (Brautbar and Williams, 2002; Brent and Rumack, 1993; Manibusan et al, 2007)
Summary
The liver is a vital organ present in vertebrates and some other animals. It has a wide range of functions such as drug metabolism, amino acid metabolism, lipid metabolism and glycolysis. Carbon tetrachloride (CCl4), a wellknown model compound for producing chemical hepatic injury and it is biotransformed by hepatic microsomal cytochrome P450 (CYP) 2E1 to trichloromethyl-free radicals (CCl3 and/or CCl3OO) (Brattin et al, 1985; Rechnagel and Glende, 1973; Rikans et al, 1994). These metabolites react with antioxidant enzymes such as glutathione (GSH) and catalase and superoxide dismutase (SOD) (Rikans et al, 1994). We investtigated the hepatoprotective and antioxidant properties of CRA used against CCl4-induced liver damage in rats
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