Abstract
Trichoderma viride Sn-9106 with high cellulase activity was used to produce enzyme on residues of Chinese herbs as substrate in solid state fermentation. Residues of Chinese herbs and peptone were found to be the best combination of carbon and nitrogen source for the production of cellulase. The nutrient composition of medium was optimized using response surface methodology. A fractional factorial design (33) was applied to elucidate the nutrient medium components that significantly affect cellulase production. The concentration of peptone and wheat bran in the medium was a significant factor. The composition of nutrient fermentation medium optimized with response surface methodology was in g/L: wheat bran, 19.8, peptone, 2.06 and KH2PO4, 2.9. Compared to the original medium, the cellulase activity increased from 3.8 to 7.5 IU/mL. Key words: cellulase, Trichoderma viride Sn-9106, response surface methodology (RSM), solid state fermentation (SSF), residues of Chinese herbs (RCH).
Highlights
IntroductionCellulase is responsible for the hydrolytic cleavage of βglycosidic bonds in cellulose and plays a critical role in the processing of lignocellulosics
In recent years, one of the most important biotechnological applications is the conversion of lignocellulosics wastes into products of commercial interest such as bioethanol (Den Haan et al, 2007; Lynd et al, 2005).Cellulase is responsible for the hydrolytic cleavage of βglycosidic bonds in cellulose and plays a critical role in the processing of lignocellulosics
Enzyme production by residue from Chinese herb of residues of Chinese herbs (RCH) contains phenolic compounds which can restrain the growth of fungi (Xu et al, 2007; Yang et al, 2009); an anti-phenol strain, T. virride Sn-9106 was isolated to produce cellulase
Summary
Cellulase is responsible for the hydrolytic cleavage of βglycosidic bonds in cellulose and plays a critical role in the processing of lignocellulosics. It is a complex made up of three classes of enzymes: exoglucanase, endoglucanase and β-glucosidase (Chandrasekharaiah et al, 2012; Salahuddin et al, 2012). Cellulase is produced by two methods: submerged fermentation and solid-state fermentation (SSF). In China, million tons of residues from the Chinese herbs are produced annually. This abundant but low value resource contains about hydrolysable cellulose
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