Abstract
Arisaema jacquemontii have shown medicinal importance and biological activities particularly, its anticancer activity, so we have decided to keep on investigating the plant in term of its chemical constituents. A chemical investigation on the chloroform extract of A. jacquemontii have resulted in the isolation of a cytotoxic, triterpenoid 2-hydroxydiplopterol (1), previously reported from the halotolerant, fungal strain aspergillus variecolor B-17. This represents perhaps the first report of cytotoxic compound from plant source. The compound (1) has been identified by multidimensional nuclear magnetic resonance (NMR) spectroscopy and comparison of its spectroscopic data with that reported for the compound. Its exhibited cytotoxicity with IC50 value of 22 µM against K 562 cell lines. The cytotoxicity properties of compound 1 were determined by the sulforhodamine B method. Key words: Arisaema jacquemontii, cytotoxic triterpenoid 2-hydroxydiplopterol, isolation.
Highlights
The genus Arisaema belongs to the family Araceae (Nisar et al, 1978)
Recent investigations have resulted in the isolation of the cytotoxic triterpenoid, 2-hydroxydiplopterol, previously reported from halotolerant fungal strain Aspergillus variecolor B17
The cytotoxicity and antioxidant properties of compound 1 were determined by the sulforhodamine B (SRB) (Skechan et al, 1990) and diphenyl-β-picrylhydrazyl (DPPH) (Chen et al, 1999) method, respectively
Summary
The genus is reputed for its physiological properties in the folk medicine system (Perry, 1965). Various parts of the plant found are used in folk medicine system and applied in different form to cure various ailments. The activity of methyl ethyl ketone and methanol extracts of the plant in rad 6 and rad 52 yeast assays have been reported (Habib et al, 1992). Recent investigations have resulted in the isolation of the cytotoxic triterpenoid, 2-hydroxydiplopterol (compound 1), previously reported from halotolerant fungal strain Aspergillus variecolor B17. To prove the reproducibility of the result, 2-hydroxydiplopterol (compound 1) was re-isolated by applying the same procedure. This represent first ever report of the cytotoxic triterpenoid 2-hydroxydiplopterol (compound 1) from plant source. The cytotoxicity and antioxidant properties of compound 1 were determined by the sulforhodamine B (SRB) (Skechan et al, 1990) and diphenyl-β-picrylhydrazyl (DPPH) (Chen et al, 1999) method, respectively
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