Abstract
In this report, a simple and rapid agar plate assay was established for screening of halophilic, inulin-degrading microorganisms. Two strains considered inulinolytic with this method were chosen and the inulinolytic activities in their culture supernatant were measured with the Somogyi-Nelson method, while their hydrolysis products of inulin were detected with TLC chromatogram. Key words: Screening, halophilic microorganism, inulinase, Lugol’s iodine solution.
Highlights
A simple and rapid agar plate assay was established for screening of halophilic, inulindegrading microorganisms
Plate screening assay is commonly used for detection of extracellular hydrolytic enzymes produced by microorganisms
Two strains considered inulinolytic with this method were chosen and the inulinolytic activities in their culture supernatant were measured with the Somogyi-Nelson method, while their hydrolysis products of inulin were detected with thin-layer chromatography (TLC) chromatogram
Summary
A simple and rapid agar plate assay was established for screening of halophilic, inulindegrading microorganisms. Plate screening assay is commonly used for detection of extracellular hydrolytic enzymes produced by microorganisms. To obtain inulin-degrading microorganisms from the environment, efficient plate screening methods are prerequisite.
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