Abstract
In all living organisms there is an increasing procession of chemical reactions and processes, which leads to fundamental internal changes. Living substance is constantly being torn down and built up. The general activities of protoplasm are being maintained. Waste products are being eliminated and vast amount of energy in constantly being expended. The sum total of these processes and changes is called metabolism. The present investigation was under taken to study the changes in organic metabolites of different regenerated stages in sugarcane var. co 740. The organic matters like chlorophyll, protein, proline, and polyphenols, reducing sugar, total sugar starch, moisture percentage and TAN were analyzed. Keywords- Sugarcane, metabolites, organogenesis, proline
Highlights
Sugarcane is one of the cash crops of India as 60% of the world sugar
Callus can be initiated from any sugarcane tissue [6].organogenesis is the development of adventitious organs are primordial from undifferentiated cell mass in tissue culture by the process of differentiation [10, 12]
It has found that addition of auxins to the culture medium to stimulate root formation .Skoog and Miller have reported that organogenesis is controlled by a balance between cytokinin and auxin.Organogenesis studies in vitro conditions helps in understanding the developmental biology of sugarcane tissue culture at metabolic and biochemical level
Summary
Sugarcane is one of the cash crops of India as 60% of the world sugar. Sugarcane industries is the second largest agro based industry [7]. Materials and methods Three different stages of tissue culture plant material were used for the investigation. Estimation of Chlorophylls Total chlorophylls were estimated by the method of Arnon [1] in which 1 gm of fresh plant material of three different stages was crushed in cold mortar using pestle in 80 %( v/v) acetone mixed with 2 ml of ammonia (25%) per liter. In other such tubes different concentration (0.5,1.0,2.0 and 4.0 ml) of standard polyphenol solution (tannic acids 0.1 mg/ml)were taken 10 ml of 2% Na2CO3 were added to each test tube to make the medium alkaline and 2 ml of Folin Denis reagent.
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