Abstract
Cat is an important experimental and pet animal and plays a key role in keeping ecological balance. Myostatin is a negative regulator of skeletal muscle growth and development in mammals, but the gene sequence of it keeps unknown in cat. To better understand the structure and function of the myostatin promoter in cat, a 1409 bp fragment containing the 5'-regulatory region of the cat myostatin gene was cloned and sequenced (GenBank accession number is GU938462). Many potential transcription factor binding sites have been found by the bioinformatics analysis, such as TATA boxes, CAAT box, E-boxes, MEF2, MEF3, MTBF, PAX3, SMAD, HBOX, HOMF and TEAF motifs. Comparative analysis for some motifs showed both conservations and differences among cat, horse, porcine and human. Key words: Cat, myostatin 5'-regulatory region, molecular cloning, sequence analysis and comparison, transcription factor binding sites.
Highlights
Myostatin, named growth and differentiation factor 8 (GDF8), was first described by McPherron and Lee (1997)
We focused on analyzing and discussing the motifs listed in Table 1, including some general core promoter elements (TATA box and CAAT box), several muscle-specific transcription factor binding sites (MTATA, MTBF, MEF2 and MEF3) and some elements playing important role in myogenesis (PAX3, SMAD, HBOX and HOMF, TEAF)
The high conservation of some motifs such as several E-boxes, MEF2, MTBF, PAX3 and SMAD among four animals further implied the importance of these motifs
Summary
Named growth and differentiation factor 8 (GDF8), was first described by McPherron and Lee (1997). It is a member of the transforming growth factor (TGF-ß) superfamily and functions as a negative regulator of skeletal muscle development and growth in mammals. Several earlier studies indicated that myostatin gene expression is regulated at the transcriptional level To better understand the structure and function of the myostatin promoter in cat, we first cloned and sequenced a 1.409 kb fragment containing the 5'-regulatory region and part of the coding sequence of the cat myostatin gene (GenBank accession number is GU938462).
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