Abstract
To investigate the protective effects of rosiglitazone on the endothelial cells in a rabbit iliac artery balloon injury, thirty male New Zealand white rabbits were randomly divided into control group (hypercholesterol diet), balloon injury group (hypercholesterol diet plus balloon endothelial denudation) and rosiglitazone group (balloon endothelial denudation plus hypercholesterol diet and rosiglitazone). Rabbits were fed for eight weeks, and balloon endothelial denudation was performed. Rosiglitazone was administrated at a dose of 0.5 mg·kg-1·d-1 from three days before surgery, and lasted for 4 weeks. Four weeks later, blood was collected and serum lipids, glucose, hs-CRP, NO, NOS and ET-1 levels were measured. Then, local iliac arteries were obtained for morphological examination and immunohistochemistry for PCNA and apoptosis. When compared with balloon injury group, the levels of serum TC, TG, LDL-C, ET-1 were significantly decreased in the rosiglitazone group. Morphological examination showed the intimal area (IA), medial area (MA) and intimal proliferation index in rosiglitazone group were significantly lower than those in the balloon injury group. The PCNA expression was markedly decreased and the apoptosis rate significantly increased in rosiglitazone group when compared with the balloon injury group. Rosiglitazone could exert protective effects on the endothelial cells after balloon injury in which the lipid-lowering, inhibited proliferation and increased apoptosis of endothelial cells, and compromised NOS activity and secretion of NO and ET-1 played important roles. Key words: Rosiglitazone, apoptosis, nitric oxide synthase, endothelin, rabbit.
Highlights
Percutaneous transluminal coronary angioplasty (PTCA) has been an ideal strategy in the treatment of coronary artery stenosis
The present study was to investigate the effects of rosiglitazone on the endothelial functions, intimal cell proliferation and apoptosis in a rabbit iliac artery balloon injury model aiming to explore the possible mechanism underlying the protective effects of rosiglitazone
Animals were housed for 1 week for accommodation and divided into 3 groups (n=10 per group): (1) control group: rabbits were fed with normal chow; (2) balloon injury group: rabbits were fed with high-fat chow and underwent iliac artery balloon endothelial denudation (Nuthakki et al, 2004); (3) Rosiglitazone group: rabbits were fed with high-fat chow, underwent iliac artery balloon endothelial denudation and treated with rosiglitazone
Summary
Percutaneous transluminal coronary angioplasty (PTCA) has been an ideal strategy in the treatment of coronary artery stenosis. Increasing studies show endothelial injury and endothelial dysfunction play critical roles in the post-PTCA restenosis (Kipshidze et al, 2004). Rosiglitazone is an anti-diabetic drug in the thiazolidinedione class of drugs. It works as an insulin sensitizer, by binding to the PPAR receptor (PPAR ) in fat cells and making the cells more responsive to insulin (Edvardsson et al, 1999). Rosiglitazone can improve lipid metabolism and exert anti-oxidative effects which play pivotal roles in the cardiovascular protection of rosiglitazone (Ceriello, 2008). The present study was to investigate the effects of rosiglitazone on the endothelial functions, intimal cell proliferation and apoptosis in a rabbit iliac artery balloon injury model aiming to explore the possible mechanism underlying the protective effects of rosiglitazone
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