Abstract
Rhizobium isolates from wild pea nodules were characterized on the basis of microbiological characteristics. P4, P7, P12, P14, P16, P19, P20, P22, P23 and P24 isolates grew at the 4.5 pH, P5, P6, P11, P12, P13, P14, P16, P17, P19, P20 and P21 isolates grew at 4% NaCl and P7, P8, P10, P11, P12, P14, P19, P20, P22, P23, P24 and P25 isolates grew at 40°C. Resistance to antibiotics (μg mL-1) was investigated in a large propotion of isolates; streptomycin sulphate (80), rifampisin (40), erythromycin (30), chloramphenicol (100), Penicillin (40). In this study, local Rhizobium bacterial isolates were isolated from wild pea root nodules and their efficacy was investigated. Isolates significantly increased plant dry matter weight. The highest nitrogen fixation was achieved with P4 inoculation. Glutamine synthetase and leghemoglobin content of the nodules were determined in the inoculated with the highest P4 isolate. © 2021 Friends Science Publishers
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