Abstract

The objective of this work was to isolate, screen and evaluate in vitro, the phosphate solubilization activity of rhizobacteria isolated from Triticum aestivum rhizosphere soil in the Meknes region of Morocco. Five best isolates from 240 ones were selected based on the solubilization of insoluble phosphates (Ca3 (PO4)2) in both agar plate and broth assays using National Botanical Research Institute's phosphate (NBRIP) medium. The bacterial isolates were identified based on their phenotypic and 16S rRNA genes sequencing data as Variovorax paradoxus (BT1), Pseudomonas reinekei (BT4), Pseudomonas libanensis (BT10), Pseudomonas lurida (BT11) and Pantoea agglomerans (BT12). P solubilization index of these isolates ranged from 2.88 to 3.48, amount of phosphate solubilized ranged from 24.23 to 56.95 mg P L-1 and drop in pH of the medium ranged from 7 to 3.2. All the isolated PSB were efficient phosphate solubilizers and can be used as bioinoculants to increase the available phosphorus in the soil for wheat plant growth. Key words: Triticum aestivum, rhizobacteria, rhizosphere, phosphate, solubilizing capacity.

Highlights

  • Environm ment and Soil Microbiology y Unit, Faculty y of Sciences, Moulay Isma ail University, Meknes, Mo orocco

  • The characterization and screening of rhizobacteria from wheat rhizosphere soil helped in the selection of various phosphate-solubilizing bacteria, have the potential to increase the available phosphate in the soil, which in turn will help to minimize the P-fertilizer application, reduce environmental pollution, promote sustainable agriculture and increase yields of wheat in Meknes area and other similar areas

  • These isolates may be the candidates for use in the saline soil

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Summary

Introduction

Environm ment and Soil Microbiology y Unit, Faculty y of Sciences , Moulay Isma ail University,, Meknes, Mo orocco. The objectiive of this work w was to isolate, scrreen and eva aluate in vittro, the phos sphate solub bilization activity of rhizobacteriia isolated from f. P solubilizatio on index of these t isolate es ranged fro om 2.88 to 3..48, amount of phosphatte solubilized d ranged from 24.23 to 56.95 mg g P L-1 and drop in pH off the medium m ranged fro om 7 to 3.2. All the isola ated PSB were efficie ent phospha ate solubiliz zers and can be used as bioinocu ulants to inc crease the available phosphorus s in the soil for f wheat pla ant growth. A deficie ency in solub ble P for ma any agrricultural soils s is one of the major fac ctors hampering crop production worldwide

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