Abstract
The D2/D3 LSU rDNA expansion segment of 13 isolates attaching tea shrubs roots in tea gardens that verified by morphological and morphometrical studies as Pratylenchus loosi Loof, 1960 from Guilan province, North of Iran, were amplified and sequenced. Amplification of the D2/D3 LSU rDNA expansion segments yielded one fragment at over all sequenced isolates as 787 bp in size. The DNA sequences were aligned using Clustral X1.81 together and with three sequences of similar region of P. loosi isolates available in Genbank database (Isolate T from Serilanka and Isolates N1 and N2 from Florida, USA). Also the genetic distance between sequences data were calculated through four methods as following; Uncorrected distance (UC), Jukes-Cantor (JC) Kimura distance (K) and Jin-Neigamma distance (JNG). For generating phyllogenetic trees both Neighbor-joining (NJ) and Unweighted Pair Group Method with Arithmetic Mean (UPGMA) were used. The results indicated that very short genetic distance exist among the Iranian isolates and between the Iranian isolates and isolate T from Serilanka whereas the Iranian isolates and isolate T were genetically distinct from isolates N1 and N2. The phyllogenetic analyses revealed relationship not only among Iranian isolates but also between Iranian isolates and isolate T.
Highlights
The tea root lesion nematode, Pratylenchus loosi Loof, 1960 is considered one of the most important and destructive pathogen attacking tea shrubs roots in tea gardens of North of Iran (Hajieghrari et al, 2005) as well as Serilanka and Japan (Sivapalan et al, 1986)
The 13 isolates showed that the qualitative characters of the populations such as number of lip annuli, spermatheca shape and tail shape agreed with original description of P. loosi Loof, 1960
It is interesting to note that all populations from Iran were replaced by G at position 320 instead of T which is present in P. loosi, isolate T
Summary
The tea root lesion nematode, Pratylenchus loosi Loof, 1960 is considered one of the most important and destructive pathogen attacking tea shrubs roots in tea gardens of North of Iran (Hajieghrari et al, 2005) as well as Serilanka and Japan (Sivapalan et al, 1986). It causes a sever decline of tea shrubs where it infects all commercial tea orchards. Intraspecific variability of certain morphological characters among genus Pratylenchus used for classical distinguishing species is well known and has been adequately documented (Roman and Hirschmann, 1969; Tarjan and Frederick, 1978)
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