Abstract
A rapid, one-step method for direct leafy and rooted bulblets regeneration of Lilium martagon var. cattaniae Vis. was established using seeds as the starting explants for in vitro culture initiation. Adventitious bulblets were regenerated from one scale explants on MS (Murashige and Skoog, 1962) medium supplied with various concentrations of plant growth regulators. The most efficient medium for multiplication was MS supplemented with 0.2 mg/l 6-benzylaminopurine (BAP) and indole-3-acetic acid (IAA) ranging from 0.25 to 2 mg/l. On average, five bulblets were obtained per explant after six weeks in culture. Upon rooting, about 200 plantlets were successfully hardened in the green house with a 95% survival rate. Preliminary experiments indicated that the plantlets from the greenhouse could be successfully used for field cultivation. In vitro propagation of this endemic and protected plant species is of great importance for germplasm conservation. Key words : Bulblet, endemic, in vitro, Lilium martagon var. cattaniae Vis., protocol.
Highlights
Lilium martagon L. belonging to the Martagon section of the large Liliaceae family is an endangered and protected species in many European countries, including Serbia (List of plants protected as natural rarities, 1993)
Considering both the percentage of explants producing bulblets and the mean number of bulblets per explant, the highest bulblet regeneration was achieved on Murashige and Skoog (MS) medium supplemented with 0.2 mg/l BAP and indole-3-acetic acid (IAA) in concentrations ranging from 0.25 to 2 mg/l (Table 2 and Figure 2B)
Medium supplemented with BAP and 0.1 mg/l naphthalene acetic acid (NAA) or medium supplemented with NAA in combination with 0.2 mg/l BAP was favorable for leaf elongation (Figure 2C)
Summary
Lilium martagon L. belonging to the Martagon section of the large Liliaceae family is an endangered and protected species in many European countries, including Serbia (List of plants protected as natural rarities, 1993). In Serbia, there are two varieties of this species: L. martagon L. var. According to Šilić (1990), variety cattaniae is a distinct species named L. cattaniae Vis. According to Šilić (1990), variety cattaniae is a distinct species named L. cattaniae Vis It is widespread in higher mountain areas of littoral Dinarides, from Mt Velebit to Mt Rumija, belonging to the Dinaridi's. Endemic species (Šilić, 1990). Preliminary results based on molecular data obtained by Sočo et al (2003) are indicative of possible bifurcation of L. cattaniae Vis. from L. martagon L
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