Abstract
Objective: This study was designed to evaluate the cytotoxicity of two resin-based cements; Bistite II DC, and Multi Bond II, after polymerization on cultured L-929 fibroblast cells.Methods: Disc-shaped samples were prepared in polyethylene molds with cylindrical cavities (5 mm diameter, 2 mm high) of BistiteII DC and Multi Bond II. After setting of resin cement disc, they were aged for 1, 3, and 5 days in Dulbecco’s Modified Eagle’s Medium (DMEM). Cell viability of L-929 fibroblast cells was assessed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and the difference between the groups was tested by analysis of variance and Tukey tests (a = 0.05).Result: After 1 day of storage, the Bistite II DC, and Multi Bond II were essentially non-cytotoxic. On days 5 of the experiment, the cell viability of two resin cements did not differ significantly (P > 0.05), but cell viability was slightly reduced on day 5. There was no significant difference in the cytotoxicity between Bistite II DC, and Multi Bond II.Conclusion: After polymerization, two resin-based cements (Bistite II, and Multi Bond) induced slight cytotoxicity. The sensitivity of cytotoxicity to L-929 cells in depended on the type of resin-based cements.
Highlights
The aesthetic aspect of dental treatment has become increasingly popular in the recent years, especially with the development of improved materials and adhesive techniques using composite resins
To evaluate the cytotoxicity of resin-based cements on cultured L-929 fibroblast cells completely, this study evaluates the cytotoxicity of two resin-based cements (BistiteII DC, and MultiBondII) after polymerization on cultured L-929 fibroblast cells
There was no significant difference in the cytotoxicity between the BistiteII DC and MultiBond II group
Summary
The aesthetic aspect of dental treatment has become increasingly popular in the recent years, especially with the development of improved materials and adhesive techniques using composite resins. It has been reported that for large cavities, indirect restorations bear advantages over direct techniques such as improvements in anatomic form, con-tour, fracture resistance and wear resistance[1]. The major goals of using resin-based adhesive materials are to enhance the bonding strength between restoration and the tooth structure, reduce the micro-leakage in the dentin–restoration interface and scatter the occlusal stress. Resin-based cement is necessary to be used for cementing non-metal prosthesis for the advantages of esthetic and strength. As resin-based adhesive materials come into close and prolonged contact withgingivo–dentin complex, their safety influence on soft tissue is of great interest, especially when the finishing line is locatedin gingival
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