Abstract
Antioxidants are used as a persuasive remedial measure against overproduction of reactive oxygen species owing to their capacity to neutralize free radicals by oxidation. Immense interest has been observed in antioxidants of natural origin and their contribution in human health. The current investigation is undertaken to explore antioxidant capacity and reducing potential of Entoban syrup and capsules. In vitro antioxidant activity was evaluated by measuring the scavenging ability of syrup and capsules on free radical 2,2’-diphenyl-1-picryl hydrazyl (DPPH). Antiradical activity assay depends on the reduction of DPPH. The reducing ability was determined by the conversion of ferric into ferrous state by antioxidant compounds. Results showed that both formulations of syrup and capsules have excellent antioxidant potential with 8.5 and 10.3 µg/ml IC50 values respectively while butylated hydroxyanisole (BHA), the standard have 5.6 µg/ml IC50 value. When formulations of syrup and capsules were compared at various concentrations (10, 50 and 100 µg/ml), reducing ability increased in a dose dependent manner just like DPPH for both formulations just like standard BHA indicting good reducing ability. In-vitro antioxidant analysis of the polyherbal drug revealed the presence of excellent antioxidant potential and reducing capability which increases in a dose dependent manner for both formulations. It might be helpful in preventing or slowing the progress of various oxidative stress- related diseases related to gastro intestinal tract. Key words: Antioxidant capacity, reducing potential, Entoban.
Highlights
Oxidative stress is a physiological condition owing to an inequity among concentrations of reactive oxygen species (ROS) and antioxidants
Results showed that both formulations of syrup and capsules have excellent antioxidant potential with 8.5 and 10.3 μg/ml IC50 values respectively while butylated hydroxyanisole (BHA), the standard have 5.6 μg/ml IC50 value
When formulations of syrup and capsules were compared at various concentrations (10, 50 and 100 μg/ml), reducing ability increased in a dose dependent manner just like diphenyl-1-picryl hydrazyl (DPPH) for both formulations just like standard BHA indicting good reducing ability
Summary
Oxidative stress is a physiological condition owing to an inequity among concentrations of reactive oxygen species (ROS) and antioxidants. ROS comprising superoxide radicals, singlet oxygen, hydroxyl radicals, and hydrogen peroxide are frequently produced as a consequence of natural reaction or due to exogenous factors (Finkel and Holbrook, 2000). Some of these ROS participate in cell metabolism including energy production, intercellular signaling and phagocytosis. Though the products of ROS-induced oxidative stress are widely used to observe their biological effects, it is significant to assess the antioxidant capability of biological fluids, cells and extracts. Antioxidants usually counterbalance radicals via a hydrogen atom transfer (HAT) or single electron transfer (SET) mechanism. SET assays enumerate the capacity of an antioxidant to transfer one electron to reduce any compound, such as free radicals, carbonyls and metals (Shulaev and Oliver, 2006)
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