Abstract
This research focuses on the activity of the desert truffle Terfezia boudieri Chatin against its associated microflora. It consists of the enumeration and pre-identification of soil microflora associated with ascocarps of T. boudieri Chatin, in contact with fungus ascocarps and at 10 cm deep. Enumeration reveals that the number of some microorganisms is higher when in contact with ascocarps of T. boudieri than at 10 cm deep. Also, bacteria are more dominant than fungi and actinomycetes (especially Bacillus sp., Pseudomonas sp., Micrococcus sp., Streptococcus sp., Proteus sp.). The number of bacteria is estimated to be 8.3 - 8.6x107 ± 0.5x107 CFU/g of dry soil, while fungal genera (with a dominance of Penicillium sp., Aspergillus sp., Mucor sp. and Alternaria sp.) are between 8.6x104 ± 0.8x104 and 4.5x105±0.8x105 CFU/g and actino-mycetes (especially Streptomyces sp.) are between 8.2x102 ± 0.3x102 and 2.2 x 103 ± 0.3 x 103 CFU/g. Microorganisms included in the nitrogen cycle are represented by free nitrogen fixing bacteria (Azotobacters) (2.2x101 ± 0.8x101MPN/g), ammonifying bacteria (4x103 ±1.0 x 101 MPN/g), nitric bacteria (between 6.8X103 ±1.0x103 and 0.6x101 ±1.0x101 MPN/g), and nitrous bacteria (between 4x101 ±1.0x101 and 0.3X101 ±1.0x101 MPN/g). Bacteria included in the degradation of organic matter are mainly proteolytic (between 1.2x106 ±0.8 x 106 and 2.4X104 ±0.8 x 104 CFU/g), amylolytic (between 6.8 x 103 ± 0.2 x 103 and 0.6 x 101 ± 0.2 x 101 CFU/g) and cellulolytic bacteria (between 1.3x104 ±0.7x104 and 1.5x103 ±0.7x103 MPN/g) but lipolytic and denitrifying bacteria are absent. These results suggest that T. boudieri Chatin stimulate soil microflora in contact with their ascocarps. Key words: Terfezia boudieri Chatin, terfez soil, microorganisms, enumeration.
Highlights
Activity of th he des sert tru uffle Te erfezia a boudiieri Ch hatin, against associ a ated s soil mic croflorra
Isolation of the dominant bacteria, actinomycetes of the soil microflora associated with Terfezia boudieri
The total number of microflora is higher in contact with ascocarps than at 10 cm deep. These results confirm those of Rougieux (1963) and indicate that the desert truffles can excrete substances that stimulate the development of these microorganisms
Summary
Activity of th he des sert tru uffle Te erfezia a boudiieri Ch hatin, against associ a ated s soil mic croflorra. Free e nitrogen fixing g bacteria (Azottobacters) were e enumerated by using the culture med dium M3 (10 g glucose, 50 ml standard saline solutio on (mixture of ssolutions A and d B mentioned above), 950 ml m distille ed water (pH 7))). Isolation of the dominant bacteria, actinomycetes of the soil microflora associated with Terfezia boudieri Isolation of bacteria and actinomycetes was performed by streaking 0.1 ml of inoculum on solid culture media in Petri dishes.
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