Abstract
The molecular diversity of Tomato spotted wilt virus (TSWV) isolates from Pakistan was investigated based on nucleocapsid protein (N) gene. It revealed that the Pakistani isolates shared 98.2–99.3% (nt) and 95.7–97.4% (aa) identities with each other and 97.2–99.3% (nt) and 92.7–97.8% (aa) with other TSWV isolates retrieved from Genbank. In a neighbour-joining phylogenetic reconstruction, four Pakistani isolates clustered with South Korean and Turkish isolates and one with Australian and South African isolates. All the Pakistani isolates shared maximum genetic diversity (0.024–0.039 and 0.026–0.029) with South African (AJ296600) and Australian (AY879109) isolates and the lowest values of genetic diversity (0.009–0.013) was recorded with Czech Republican isolate (AJ296599). The gene differentiation co-efficient value (Fst) was 0.33 less than standard value noticed as 0.05145, between globally reported and Pakistani TSWV isolates considering it a frequent gene flow. The significant values of statistical tests based on three genetic differentiation analysis; Ks⃰; 4.86231, Z; 34.27778 and Snn; 0.55556 were observed. The negative values were recorded according to statistical test as Fu, & Li’s D ⃰ (-2.55465), Fu, & Li’s F ⃰ (-2.78103) and one positive value from Tajima’s D (2.10697). These results indicate low polymorphism frequency in TSWV population of Pakistan. © 2021 Friends Science Publishers
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