Abstract
Barbiturases exist solely in bacteria and encompass an undistinguished protein family. s-Triazine compound introduction into the environment owing to recent industrial practices have revitalized barbiturases. Codon usage patterns were analysed in this study for 17 barbiturase genes encoded by 16 bacterial species. Multivariate and correspondence study of amino acid and codon usage was employed for detecting the cause of variation in the gene content. GC3 of synonymously variable codons, RSCU, NC and CAI were estimated with statistical softwares. Examination of DNA composition along with codon usage was done to reveal dynamics of gene evolution and expression of this enzyme. Keywords: Codon usage, barbiturase gene, multivariate statistical analysis African Journal of Biotechnology , Vol. 13(2), pp. 194-201, 8 January, 2014
Highlights
Codon penchant arises in the majority species across all kingdoms and shows a discrepancy in genes of same and dissimilar species (Duret, 2002)
It is anticipated that meagre biasness or none at all in distribution of A, T, G- and Cending codons exists in the barbiturase genes
Nc values vary in range from 20 to 61
Summary
Codon penchant arises in the majority species across all kingdoms and shows a discrepancy in genes of same and dissimilar species (Duret, 2002). The possible reasons effecting codon usage pattern are mutations, genetic drift, translational selection and horizontal gene transfer (Ermolaeva, 2001). Comparative sequence analysis is a potent approach for detecting mutations, selection methods and finding rationale of biased and unbiased gene changes and evolutionary processes. Sequence information has been used to infer gene location effect on codon usage for a few prokaryotic species (Sharp and Matassi, 1994). Comparative homologous gene sequence analysis within same and different microbial species facilitates the accomplishment of this task and assists in exposing footprints left by evolution. Preference of codon usage and its quantification facilitates evolution compre-
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
