Abstract
Terfizia claveryi was examined for in vitro antibacterial activity using the disc diffusion, well diffusion method, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). T. claveryi exhibited excellent antibacterial activity against all clinical isolates of corneal ulcer tested, especially against Pseudomonas aeruginosa which showed the maximum antibacterial activity with mean zone of inhibition 20.33 mm at concentration of 100 mg/ml. The MIC for Staphylococcus aureus ranged from 0.040-1.250 mg/ml and MBC for Escherichia coli was 75 μl/ml. In the present study, the MIC value of the active aqueous extract were lower than the MBC values suggesting that, T. claveryi aqueous extracts were bacteriostatic at lower concentration but bactericidal at higher concentration. Also, the bacterial zone of inhibition increased with the increasing concentration of T. claveryi aqueous extract. To the best of our knowledge, this is the first report for the novel antibacterial activity of T. claveryi aqueous extract. This active compound may be used as alternative therapeutic drug for the control of corneal infections. However, further research is needed to examine its in vivo mechanism of action, toxicity, and therapeutic effect. Key words: Bacteria, corneal infection, antimicrobials, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), Terfizia claveryi
Highlights
In the 21st century antibiotic resistance of clinical bacterial isolates are increasing drastically, the search for new and safe anti-bacterial compounds are important and natural medicinal products seems to be a logical and effective source for seeking new antimicrobial agents
All the tested bacteria were sensitive to T. claveryi aqueous extract
This study reveals that the P. aeruginosa was highest susceptible bacteria with 20.33 mm zone of inhibition followed by S. aureus (19.00 mm), S. epidermidis (18.00 mm), S. faecalis (17.00 mm), E. coli (15.33 mm), P. vulgaris (15.33 mm) and K. pneumonia (14.66 mm) at the test concentration of 100 mg/ml, which was comparable to standard antibiotic ciprofloxacin 5 mg/disc (Table 1)
Summary
In the 21st century antibiotic resistance of clinical bacterial isolates are increasing drastically, the search for new and safe anti-bacterial compounds are important and natural medicinal products seems to be a logical and effective source for seeking new antimicrobial agents. T. claveryi ascocarps contain 16% protein, 28% total carbohydrates, 4% total crude fiber, 2% total crude fat and rich in mineral as well as carbohydrate contents, with nine saturated and four unsaturated fatty acids and 29 amino acids, unique flavor, nutritional value and medicinal properties for a variety of ailments (Al-Delaimy, 1977). It is used as a nourishing and invigorating preparation for convalescents in Mediterranean countries (Janakat et al, 2004). The medicinally important T. claveryi fruit was selected in this study to investigate whether having antimicrobial activity against clinical isolates of corneal ulcer
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
