Abstract
Amplified fragment length polymorphism (AFLP) and inter-simple sequence repeat (ISSR) markers were used to study the DNA polymorphism in elite mungbean genotypes. A total of nine AFLP primer combination and 22 ISSR primers were used. Amplification of genomic DNA of the 30 genotypes, using AFLP analysis, yielded 300 fragments that could be scored, of which 192 were polymorphic, with an average of 21.3 polymorphic fragments per primer. Number of amplified fragments with AFLP primers ranged from 29 (E-AAC: M-CAG) to 10 (E-ACG: M-CAT). Percentage polymorphism ranged from 46.3% (E-AAC: M-CCA) to a maximum of 100% (E-AAC: M-CAC), with an average of 64%. The 22 ISSR primers used in the study produced 108 bands across 30 genotypes, of which 68 were polymorphic. The number of amplified bands varied from two UBC820) to ten URP 6F). The average numbers of bands per primer and polymorphic bands per primer were 4.9 and 3.1, respectively. Percentage polymorphism ranged from 25% (UBC844) to 85% (UBC846, UBC864, UBC895), with an average percentage polymorphism of 58.3% across all the genotypes. AFLP markers were more efficient than the ISSR assay, as they detected 64% polymorphic DNA markers in Vigna radiata as compared to 58.3% for ISSR markers. The Mantel test between the two Jaccard's similarity matrices gave r = 0.19, showing low correlation between AFLP- and ISSR-based similarities. Clustering of genotypes within groups was not similar when AFLP and ISSR derived dendrograms were compared. Key words: AFLP, ISSR, Vigna radiata (mung bean), marker index, unweighted pair-group method with arithmetic averages (UPGMA).
Highlights
Mungbean is a widely grown food grain legume in the developing world
All the 23 Inter simple sequence repeat (ISSR) markers were successfully amplified across the 30 mungbean genotypes but only 22 markers were polymorphic (Table 2)
Genetic variation was detected among 30 genotypes of mungbean using the ISSR and AFLP marker techniques
Summary
Mungbean is a widely grown food grain legume in the developing world. It is cultivated almost in all the four seasons in one state or the other state of India. Out of the 16 essential nutrients, seven are classified as micronutrients or trace elements Out of these seven micronutrients, iron and zinc play very important and vital role in animals, humans and plants health and development. It improves the soil fertility by fixing atmospheric nitrogen into available form with the help of Rhizobia species present in the nodules of its roots (Ashraf et al, 2003). Pedigree analysis of released varieties of mungbean revealed that only the top three to ten ancestors contributed 30 to 79% to the genetic base (Katiyar et al, 2007, 2008)
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