Abstract
Seven microsatellite markers OarAE101, BM1329, OarHH55, BM143, BMS2508, OarAE129 and OarFCB11 closely associated with high reproduction trait in sheep were analyzed for polymorphisms in Xinong Saanen dairy goat. The results indicated that there were high genetic polymorphisms at six microsatellite loci. The number of effective alleles (Ne), polymorphism information content (PIC) and average heterozygosity (He) were the highest at OarFCB11 and the lowest at OarAE129 in Xinong Saanen dairy goat. The analysis of the effect of the six polymorphisms microsatellite loci on the litter size of Xinong Saanen dairy goat indicated that these polymorphisms microsatellite loci had positive effect on the litter size. Key words: Microsatellite markers, Xinong Saanen dairy goat, genetic polymorphism, litter size.
Highlights
Microsatellites, known as simple sequence repeats, are long stretches of a short (1 to 6 bp) tandemly repeated DNA unit, such as the motif CAA repeats 20 times
We can see that 6 microsatellite loci have polymorphisms in Xinong Saanen dairy goat from figures 1 to 7 except for the BMS2508 microsatellite locus
The allele frequencies of 6 polymorphisms microsatellite loci In Xinong Saanen dairy goat, 8 alleles of OarAE101 microsatellite locus were found with fragment size from 109 to 137 bp, the maximum frequency is 0.25 of 114 bp; 7 alleles of OarAE129 microsatellite locus were found with fragment size from 155 to 205 bp, the maximum frequency is 0.3878 of 205 bp; 10 alleles of BM1329 and OarHH55 microsatellite loci were found, with fragment size from 182 to 234 bp and 125 to 170 bp at BM1329 and OarHH55 microsatellite locus, respectively, the maximum frequency is 0.1480 of 225 and 190 bp a BM1329 microsatellite locus and 0.1837 of 165 bp at
Summary
Microsatellites, known as simple sequence repeats, are long stretches of a short (1 to 6 bp) tandemly repeated DNA unit, such as the motif CAA repeats 20 times. Reproductive traits, which are under the control of multiple genes, are economically important traits in livestock. Known as litter size, is of special meaning in the selection of animals with high reproductively, especially for the animals raised by people as economic source. It is hard to use normal breeding methods to improve litter size and the maker-assisted selection may accelerate the goat breeding process
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