Abstract
Screening of new microbial strains for enzyme production is a key step in the improvement of industrial bioprocesses. The objective of this work was to isolate fungal strains from Brazilian rainforests and to evaluate their potential to produce industrial enzymes by solid state fermentation. First, 131 strains of filamentous fungi were isolated from Amazon rainforest and Atlantic rainforest regions. Strains selected by simple plate assay had their ability to produce lipase and tannase by fermentation of wheat bran evaluated. Maximum enzymatic activity of 1.35 and 18.7 U/mL were reached by lipase and tannase, respectively. The strain IB28a was identified by molecular and morphologic techniques as Colletotrichum sp. Lipase produced by this strain reached specific activity of 25.97 U/mg and showed Km and Vmax of 6.3 and 19.5, respectively. Lipase from Colletotrichum sp. had an optimal temperature range of 25 to 35°C and optimal pH range of 6.5 to 7.0. Moreover, the enzyme was stable after 1 h at temperatures up to 40°C and after 24 h at pH 6.5. Key words: Bioprospection, filamentous fungi, lipase, tannase.
Highlights
Sc creenin ng of fiilamen ntous ffungi from Brazilian rainfores sts for enzym me productio on
This work is aimed at isolating new strains that are capable of producing the extracellular lipases and tannases enzyme by solid state fermentation (SSF) from two Brazilian rainforests, as well as, to identify the selected microorganisms and to biochemically characterize the produced enzymes
Tannase enzymatic activity was estimated by the enzymatic index (EI), which was determined by the ratio between the halo diameter and the colony diameter (Hankin and Anagnostakis, 1975)
Summary
Fo ood Science Department, School of Foo od Engineering, University of Campinass, 80 Rua Mon nteiro Lobato,, postal Code. The obje ective of this s work was to t isolate fun ngal strains from Brazilian rainfores sts and to evaluate their potential to produce industrial enzymes by s solid state fe fermentation. Progress had been achieved in the last years as a result of the efforts in isolation of newly strains with higher tannase production, the optimization of fermentation systems and the development of economically viable purification methods (Chávez-González et al, 2012). In a non-aqueous medium, lipase can catalyze the synthesis of acylglycerol from free fatty acids and glycerol (Macrae and Hammond, 1985). This work is aimed at isolating new strains that are capable of producing the extracellular lipases and tannases enzyme by solid state fermentation (SSF) from two Brazilian rainforests, as well as, to identify the selected microorganisms and to biochemically characterize the produced enzymes
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