Abstract

To study the pattern of genetic diversity in 45 genotypes of common bean, 19 RAPD primers were used. Of 253 bands produced, 236 bands (94.22%) were polymorphic in which maximum number (20 polymorphic bands) were observed in the profiles of the primer OPB-07. Highest PIC value (0.79) was observed for the primers OPG-14 and OPE-1, whereas lowest PIC value (0.34) was recorded for the primer OPA-11 with an average PIC value of 0.54. Similarly, highest effective multiplex ratio value (0.11) was scored for the primers OPA-04 and OPG-6 and the lowest value (0.05) was recorded for the primer OPB-07. Primer OPG-14 and OPE-1 exhibited the highest marker index value (0.078) and the lowest value (0.024) was recorded for OPA-11. Pair-wise genetic similarity coefficients of genotypes varied from 0.56 to 0.92 with an average of 0.70 amongst 45 genotypes. The maximum similarity coefficient (0.92) was noticed between G-9 and G-8. Cluster analysis separated 45 genotypes into seven major clusters which were further grouped into various sub-clusters. Among these seven clusters, the maximum number of genotypes (12) were recorded in cluster I, while cluster VII formed a mono-genotypic cluster.    Key words: Genetic diversity, polymorphism, cluster analysis, random amplified polymorphic DNA markers (RAPD), common bean. &nbsp

Highlights

  • The common bean (Phaseolus vulgaris L.) belongs to the family Leguminosae, subfamily Papilionoideae, and tribePhaseoleae

  • The present investigation was undertaken with an objective to assess genetic diversity in common bean genotypes using random amplified polymorphic DNA markers (RAPD) markers

  • Polymorphism information content (PIC) or expected heterozygosity scores for each RAPD marker was calculated based on the formula: n

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Summary

Full Length Research Paper

Examination of genetic diversity in common bean (Phaseolus vulgaris L.) using random amplified polymorphic DNA (RAPD) markers. To study the pattern of genetic diversity in 45 genotypes of common bean, 19 RAPD primers were used. Highest PIC value (0.79) was observed for the primers OPG-14 and OPE-1, whereas lowest PIC value (0.34) was recorded for the primer OPA-11 with an average PIC value of 0.54. Was scored for the primers OPA-04 and OPG-6 and the lowest value (0.05) was recorded for the primer. Primer OPG-14 and OPE-1 exhibited the highest marker index value (0.078) and the lowest value (0.024) was recorded for OPA-11. Cluster analysis separated 45 genotypes into seven major clusters which were further grouped into various sub-clusters Among these seven clusters, the maximum number of genotypes (12) were recorded in cluster I, while cluster VII formed a mono-genotypic cluster

INTRODUCTION
Primer name
The research was carried out at the Experimental Farm of Urban
DNA extraction and PCR
Percentage of polymorphism
Polymorphism information content
Cluster analysis
Effective multiplex ratio
Name of genotypes
Full Text
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