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Abstract
The objective was to develop an optimal, albeit low-cost medium for bacteriocin production, thereby facilitating industrial production. Soybean meal and peptide, two low-cost nitrogen sources often applied in industrial fermentation, were used to replace their expensive counterparts in De Man Rogosa and Sharpe (MRS). Two factors were first chosen from the 11 considered in the Plankett-Burman (PB) design. Then, the path of steepest ascent and central composite design (CCD) were used to approach the optimum region of the response and determine the maximum activity of the bacteriocin. Optimal concentration of glucose (36.3 g/l) and NaCl (1.41 g/l) stimulated the production of bacteriocins. And the optimal equation was then verified by 50 L fermentor. Under optimized conditions, Lactobacillus plantarum YJG produced a 1.4 fold higher production of bacteriocin than the common MRS, with 40.6% cost savings relative to non-optimized conditions. Key words: Lactobacillus plantarum YJG, bacteriocin, response surface methodology, central composite design, optimization.
Highlights
Bacteriocins, ribosomally synthesized antibacterial peptides, are regarded as potential alternatives to conventional antimicrobials (Klaenhammer, 1988; Cleveland et al, 2001)
The bacteriocin produced by Lactobacillus plantarum YJG in this study inhibited some strains of pathogenic bacteria, including Staphylococcus aureus and Salmonella typhi (Integrated Vocational Development Centre, IVDC, Beijing, China)
Glucose was remained the best carbon source; yeast extract, soybean meal and peptide were all chosen as the proper nitrogen sources for the high bacteriocin production and biomass (Figure 2)
Summary
Bacteriocins, ribosomally synthesized antibacterial peptides, are regarded as potential alternatives to conventional antimicrobials (Klaenhammer, 1988; Cleveland et al, 2001). The bacteriocin produced by Lactobacillus plantarum YJG in this study inhibited some strains of pathogenic bacteria, including Staphylococcus aureus and Salmonella typhi (Integrated Vocational Development Centre, IVDC, Beijing, China). Bacteriocins were one family of microbial defense system, which meant they may prohibit the invasion of other strains or the change of the environment, both biotic and abiotic (Riley and Wertz, 2002). Many kinds of bacteriocins have been found from different bacteria; only one bacteriocin named nisin has been really applicated. Most of other bacteriocins are focused on isolation and purification on a laboratory scale, with little or no consideration to industrial-scale production or being applicated to human life
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