Abstract
This present study attempted to identify the antibacterial effects of aqueous and gaseous ozone in human root canals that are contaminated with Pseudomonas aeruginosa. The differences and antibacterial effects of gaseous and aqueous ozone (manual and ultrasonic) have not been compared to each other yet in any studies. Eighty single-root premolar teeth were prepared and then disinfected and sterilized. P. aeruginosa were incubated in root canals and kept at 37°C for 24 h. The root canals were contaminated with P. aeruginosa divided into 1 positive control, sodium hypochlorite (NaOCl) and three experimental groups: aqueous ozone with manual technique, aqueous ozone with ultrasonic technique and gaseous ozone (n=10). Disinfection procedures were performed for 5 min in order to ensure standardization among all working groups. Remaining microorganism colonies were counted on blood agar plates. Then, data were evaluated and statistically analyzed by one-way ANOVA and Tukey’s test. As a result, although there were no statistically significant differences between the three groups (NaOCl, aqueous ozone with manual, and ultrasonic technique) (P>0.05), there were statistically significant differences between the gaseous ozone and all other groups (P<0.05). Both the manual and ultrasonic techniques of aqueous ozone achieved complete elimination of P. aeruginosa in root canals. Key words: Gaseous ozone, aqueous ozone, microorganisms.
Highlights
The fundamentals for root canal treatments are the removal of the remaining pulp tissue, dentinal debris, and the elimination of microorganisms with chemomechanical preparation from the root canal system
These investigations found that a high concentration of NaOCl (5.25%) became sufficient for complete sterilization in root canals contaminated with P. aeruginosa (Huth et al, 2009; Baumgartner and Cuenin, 1992; Piccolomini et al, 2002)
5.25% NaOCl was applied as a negative control group to compare the antibacterial efficacy of experimental groups against P. aeruginosa in root canals
Summary
The fundamentals for root canal treatments are the removal of the remaining pulp tissue, dentinal debris, and the elimination of microorganisms with chemomechanical preparation from the root canal system. Pulpal and periapical inflammation occurred because of pathogen microorganisms (Siqueira, 2002). One of the mostly eliminated microorganisms from root canals is Pseudomonas aeruginosa. Some studies reported that P. aeruginosa was isolated as an endodontic infectious agent. Its morphology is highly similar to other gramnegative rods commonly found in endodontic infections (Ranta et al, 1988; Haapasalo et al, 1983). Various irrigation solutions have been used during chemomechanical preparation to eliminate or reduce the number of microorganisms in endodontics. An ideal endodontic irrigant should be antimicrobial, nontoxic to periapical tissues, capable of dissolving tissue or debris, able to lubricate the canal, and improve the
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