Abstract
Subtilisins (E.C.3.4.21.62) are alkaline proteases that are secreted by members of the genus Bacillus. They are serine proteases that exhibit high specific activity on proteinaceous substrates, function optimally at moderate temperatures, and are stable under alkaline conditions. Thus the use of subtilisin as an enzyme additive could help in development of quality laundry detergents. In this work the subtilisin production from Bacillus subtilis (MTCC 441) was improved by altering and optimizing the media components. This alteration was brought up by process development strategy. The effect of yeast extract, casein, peptone and sodium chloride on subtilisin production was studied and were optimized using Box Behnken Design. The optimal growth conditions for B. subtilis MTCC 441 were found to 37oC, and pH 7.5. The optimal media composition for subtilisin production was found to be yeast extract at 6.75 g/L, peptone at 4.41 g/L, sodium chloride at 6.08 g/L, casein at 10.75 g/L with glucose at 5 g/L. The predicted and observed response were 181.00 U/mg (with desirability =0.87) and 185.70 U/mg, respectively. Key words: Bacillus subtilis, subtilisin, Box-Behnken design, media optimization.
Highlights
The major extracellular proteolytic enzymes secreted by microbes are neutral and alkaline serine proteases, such as subtilisin produced by Bacillus subtilis
Initial growth studies on B. subtilis MTCC 441 were carried out using nutrient medium
The culture was grown at different temperature and pH to analyze the effect of temperature and pH on the growth and subtilisin production
Summary
The major extracellular proteolytic enzymes secreted by microbes are neutral (metallo-) and alkaline serine proteases, such as subtilisin produced by Bacillus subtilis. Subtilisin is one of the most extensively studied of all bacterial proteins, due to its commercial importance (Ward, 1985) This protease is produced at the onset of sporulation and a complex network of genes and regulators, which regulates the transition state of protein (Kalisz, 1988), controls the expression. Proteases account for approximately 40% of the total enzyme sales in various industrial market sectors, such as detergent, food and pharmaceutical industries (Beg et al, 2003; Shang and Yang, 1999) Their importance is illustrated by the fact that the amount of subtilisin produced and used in the European Union in 2002 was 900 tons of pure enzyme Subtilisins. We have attempted to optimize the production of subtilisin from B. subtilis MTCC 441 using BoxBehnken design
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
