Abstract
The chemical compositions of the essential oil of Asperula oppositifolia aerial parts were examined by gas chromatography/mass spectrometry (GC/MS); forty-two compounds were identified. Analysis of this essential oil revealed the presence of 2-(6,6-Dimethyl bicyclo[3.1.1]hepta-2en-2-yl) ethanol (17.16%), Decane (8.47%) , Dibuthyl phthalate (5.59%) and 1-Bromo Naphthalene(4%). The antioxidant activity of aerial parts of methanolic extract was studied by in vitro 2’2’-diphenylpicrylhydrazyl (DPPH) radical – scavenging activity, revealing that this plant could be used as new medicinal resource for antioxidant agent. Key words: Chemical composition, essential oil, 2-(6,6-Dimethyl bicyclo[3.1.1]hepta-2en-2-yl)ethanol, Asperula oppositifolia ,antioxidant activity.
Highlights
Asperula oppositifolia rechingeri is described from NorthKhorasan Province, Iran
The various compounds were identified by comparison of their Kováts retention indexes, determined utilizing a non-logarithmic scale on nonpolar (Rtx-5MS) columns, and by comparison of the mass spectra of each GC component with those of standards and reported literature (Jennings et al.,1980)
High resolution gas chromatography-mass spectrometric (HP GC-MS) analysis and Kováts Index values showed that its principal components are:2-(6,6Dimethyl bicyclo[3.1.1]hepta-2en-2-yl)ethanol (17.16%), Decane (8.47%), Dibuthyl phthalate (5.59%) and 1Bromo naphthalene(4%) and Dodecane (3.08%)
Summary
Asperula oppositifolia rechingeri is described from NorthKhorasan Province, Iran. A.oppositifolia comprises six subspecies mainly distributed in E. Describing these taxa originating from Iran demonstrates the extent of diversity of Asperula species in this country. Morphological evidence supports taxonomic position of these taxa in A.oppositifolia, and the subspecies appear to be most closely related to subsp pseudo-cynanchica Ehrend (Ghahramaninezhad et al, 2006). Phytochemical studies on this plant have been carried out, and flavonoids were identified (Borisov et al, 1972)
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