Abstract
A micropropagation method is described for banana ( Musa Spp .) Cv. Grand Naine. Suckers were surface sterilized with HgCl 2 (0.1%) for 6 min which gave minimum contamination with maximum culture establishment. Of various treatment combinations, Murashige and Skoog (MS) medium + BAP 4.00 mg/l with IAA 2.00 mg/l resulted in maximum establishment of cultures in lesser time. MS medium + BAP 4.00 mg/l + IAA 2.00 mg/l gave maximum multiple shoots. Maximum rooting was obtained on MS medium (half strength) supplemented with IBA 1.00 mg/l and activated charcoal 200 mg/l. Keywords: Grand naine, micro propagation, murashige and skoog medium, Musa spp. and suckers. African Journal of Biotechnology , Vol 13(27) 2696-2703
Highlights
Banana is a perennial herbaceous monocot which belongs to Musa genus of the Musaceae family
Data recorded for different parameters were subjected to completely randomized design (CRD)
Huge number of explants die due to microbial contamination; it studies that contamination free culture were established by HgCl2 for 6 min followed by several washes in sterile water and obviated the need to develop extensive and complicated surface sterilization protocol, Suneeta and Das (2008) studied that the investigation on the effect of surface sterilization agents showed variation in respect to their sterilizing property; out of sterilizing chemicals, calcium hypochlorate, hydrogen peroxide and bromine water did not show any good response and Jaisy and Ghai (2011) who worked on in vitro propagation of banana found treatment of explants with HgCl2 (0.1%) for 6 min most effective surface sterilization procedure registering maximum culture establishment with minimum contamination
Summary
Banana is a perennial herbaceous monocot which belongs to Musa genus of the Musaceae family. Murashige and Skoog (MS) medium + BAP 4.00 mg/l with IAA 2.00 mg/l resulted in maximum establishment of cultures in lesser time.
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