Abstract
The objective of this study was to evaluate the production of embryogenic calluses and plant regeneration of seven popcorn inbred lines (Zea mays L.) in order to identify lines for further transference of genes to a generation of transgenic plants. The N6 medium with supplementation was used to induce calluses in immature embryos, and supplemented MS medium was used to stimulate plant regeneration of P11, P12, P15 and P18 inbreed lines. The number of regenerated plants was the highest for the P15 inbred line. The P15 popcorn inbred line represents a potential genotype for transferring plant regeneration competence to other popcorn genotypes. Key words: Maize, immature embryos, callus induction, somatic embryos, regeneration competence.
Highlights
Somatic embryogenesis using immature embryos for obtaining in vitro regenerated popcorn plants (Zea mays L.) has been established since the 1980s (Green, 1982; Vasil and Vasil, 1986; Prioli and Silva, 1989)
The objective of this study was to evaluate the production of embryogenic calluses and plant regeneration of seven popcorn inbred lines (Zea mays L.) in order to identify lines for further transference of genes to a generation of transgenic plants
There are no reports on scientific literature of somatic embryogenesis and plant regeneration in popcorn genotypes
Summary
Somatic embryogenesis using immature embryos for obtaining in vitro regenerated popcorn plants (Zea mays L.) has been established since the 1980s (Green, 1982; Vasil and Vasil, 1986; Prioli and Silva, 1989). For more than three decades, obtaining regenerated corn plants via somatic embryogenesis has been considered a relatively complex procedure. The induction of embryogenic callus, as well as their potential to regenerate whole plants has been dependent on the corn genotype, and the adaptation of specific protocols has been an inevitable and usual procedure (Binott et al, 2008; Rakshit et al, 2010; González et al, 2012). Several efforts have been made to identify regenerated plants in genotypes with agronomic traits of interest, hybrids, and popcorn inbred lines from different breeding programs. There are no reports on scientific literature of somatic embryogenesis and plant regeneration in popcorn genotypes.
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