Abstract
Stress, excessive consumption of processed foods, pollutants and many other factors of today's life have caused oxidative stress in our body and damage to genetic material in our cells, leading to diseases such as diabetes, hypertension, cancer, metabolic syndrome, among others. Medicine has returned to take up traditional remedies mainly in plants. Chaya (Cnidoscolus aconitifolius) has been studied in this regard, mainly for its antidiabetic effects; however, there is little information about its antioxidant and protective properties against genotoxic damage. The present study focuses on the evaluation of the antioxidant and protective properties to genotoxic damage of aqueous and methanolic extracts of chaya leaves (C. chayamansa) in a model in mice which were induced to oxidative stress and genotoxic damage by exposure to arsenic and low doses of streptozotocin. Genotoxic damage was assessed by micronucleus count and antioxidant properties were assessed in the extracts and plasmas of the mice by the ABTS radical and using Trolox as standard. Additionally, the extracts were analyzed by HPLC-MS/MS. Mice treated with aqueous extract showed better recovery than those treated with methanolic extract; however, no significant statistical differences were found between both groups. HPLC analysis suggests the presence of some compounds of interest such as ferulic acid, protocatechonic acid, riboflavin, kaempferol and beta carotene. These could be associated with the effects of protection against genotoxic damage and as antioxidants found in the study. The aqueous extract seems have the best performance against genotoxic damage and have better oxidative properties, so the consumption of chaya can be recommended. Key words: Cnidoscolus aconitifolius, Chaya, genotoxic damage, oxidative stress, micronucleus, High Performance Liquid Chromatography (HPLC).  
Highlights
Genotoxic damage caused by overprocessed food consumption, current life stress, pollution etc. cause serious damage to the genetic material of the cells, which can end in severe complications such as cancer development and serious mutations
The present study aimed to evaluate the antioxidant and protective effect against genotoxic damage in Long Evans rats by ingestion of aqueous or methanolic extracts of leaves of C. aconitifolius
The ethical guidelines established by the Mexican standard NOM-062-ZOO-1999 (2001) that specifies the care of laboratory animals were respected throughout the whole experiment and were certified by the MVZ Gerardo del Campo Galindo
Summary
Beneficial effects are attributed to it in the treatment of diabetes, improving blood circulation, treating varicose veins, anemias and protective properties against genotoxic damage. Little has been published of its antioxidant and protective properties against genotoxic damage (Sánchez and Méndez, 2013). We currently know that some compounds can lead to the formation of reactive oxygen species (ROS) such as the OH radical and the peroxides, causing the alteration of the oxide-reduction homeostasis inside the cell (Sánchez and Méndez, 2013; García et al, 2013). Genotoxic damage caused by overprocessed food consumption, current life stress, pollution etc. The present study aimed to evaluate the antioxidant and protective effect against genotoxic damage in Long Evans rats by ingestion of aqueous or methanolic extracts of leaves of C. aconitifolius
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
