Abstract
In order to promote the growth of photosynthetic bacteria (PB) use as biofertilizers in paddy fields, this study conducted during 2011 to 2012 at Department of Agricultural Microbiology, University of Agricultural Sciences, Dharwad, Karnataka, India. Twenty phototrophic purple non-sulphur bacterial (PPNSB) isolates were isolated from paddy rhizosphere soils of Northern Karnataka. Isolates were tested by morphologically, biochemically and physiologically according in to Bergey’s manual. All the isolates belong to PPNSB Rhodobacter sp. In vitro nitrogen fixation was estimated by percent nitrogen content in cells which were grown on nitrogen free culture broth. Maximum percent nitrogen content was recorded in culture broth inoculated with isolate NKPRSR -1 (2.50%). Based on percent nitrogen content, the best five efficient isolates were selected for hydroponic culture to see the effect on paddy seedlings growth under laboratory condition. In hydroponics Complete Randomized Design was used to accommodate 13 treatments with 5 replication, with the combination of nitrogen source and without nitrogen sources. Results revealed that treatment having isolate Rhodobacter sp. NKPRSR 1+ Nitrogen source show maximum plant height (26.67 cm), root length (13.17 cm), dry weight (16.50 mg/ plant) and percent nitrogen content (0.99%) after 30 days of sowing. Key words: Isolation, purple nonsulphur bacteria, paddy, hydroponics, rhodobacter, rhizosphere.
Highlights
IIsolatio on of potenti p ial pho ototrop phic pu urple n non-su ulphur b bacteriia in pa addy and a the eir effe ects on n padd dy seed dlings in hydroponic culturre
In order to promote the e growth of photosynthet p tic bacteria ((PB) use as b biofertilizers s in paddy fie elds, this study cond ducted durin ng 2011 to 2012 at Department o of Agricultu ural Microbio ology, Unive ersity of Agricultural Sciences, Dharwad, Karnataka, K
Max ximum percent nitrogen n content was record ded in culturre broth inoc culated with isolate NKP
Summary
The logarithmic growth phase cells were centrifuged and the cell pellets were washed twice with sterilized distilled water and resuspended in phosphate buffer 0.05 M, pH = 7 After these starved bacterial cells will be referred to as inoculums. Contaminant-free uniformly germinated seeds were aseptically transferred to sterilized growth assemblies containing 600 ml of the sterile nutrient solution with or without nitrogen. At the end of the 30th day experimental period, the plants were removed gently from the assemblies and washed in distilled water, the shoot and root portions were separated and the growth measurements were taken. Multiple mean comparisons were made by Duncan Multiple Range Tests using MSTAT- C software
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