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Abstract
More than 80% of the plant diseases are caused by fungi. Usually, fungi not only destroy the plants, but also produce mycotoxins that are harmful to human health. At present, chemical fungicides are mainly used for the prevention of fungi-related plant diseases, however, research and development of biological prevention and controlling are of great importance. In this work, the effects of pH and temperature on cell growth and CF66I formation in batch culture of Burkholderia cepecia CF-66 were studied. The pH value has a marked effect on cell growth and production of CF66I. The lag phase was much longer when pH set lower (e.g.5.0) or higher (e.g.8.0). For earlier phase, optimal pH value was 6.0, because the lag phase can be shortened and the whole fermentation phase can also be shorten and then quickly goes into CF66I production phase. In the late phase, the higher pH is in favor of the production of CF66I. Different temperature have different effect on cell yield, specific growth rate, CF66I yield and specific synthesis rate. In the prophase of fermentation, it is better to set higher temperature to make the cell growth maximizing as soon as possible. However in mid-anaphase, lower temperature shortens the fermentation time, reduce heating energy and the cost. According about results, an optimal control strategy was constructed.
Highlights
Every year, plant-pathogenic fungi such as Fusarium sp., Pythium sp. and Rhizoctonia solani cause millions of dollars worthy of crop damage all over the world despite the extensive use of chemical pesticides
Time course of CF66I production at pH 6.0 was different from that at pH 5.0, the lag time of cell growth was the shortest than all tested pH value, DCW reached the maximum value of 3.999 g/L at 32 h, while the maximum activity of CF66I was obtained at 48 h
Time course of CF66I production at pH 7.0 and 8.0 were similar, the difference was that the maximum activity (8.166 U/ml) of CF66I at pH 8.0 is higher than that at pH 7.0 (6.996 U/ml)
Summary
Plant-pathogenic fungi such as Fusarium sp., Pythium sp. and Rhizoctonia solani cause millions of dollars worthy of crop damage all over the world despite the extensive use of chemical pesticides. Plant-pathogenic fungi such as Fusarium sp., Pythium sp. Many reports have been published concerning to new antibiotics having biocontrol activity including some kinds of bacteriocins, alkaloids, lipopeptides and polypeptide (Arima et al, 1968; Cupples and Sen, 1978; Wakayama et al, 1984; EI-Banna and Winkelmann, 1998). Some antibiotics, such as polyoxin, validamycin, amipurimycin and abomycin, have been extensively used in agricultural biocontrol (Cabib et al, 1991; Yu et al, 2005). The inhibitory efficacy against soilborne plant pathogens is associated with the productivity of antibiotics, in general the productivity is low because they are secondary metabolites
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