Abstract
To study the genetic and phylogenetic relationship of gobioid fishes in China, the representatives of 10 gobioid fishes from 2 subfamilies in China were examined by amplified fragment length polymorphism (AFLP) analysis. We established 220 AFLP bands for 45 individuals from the 10 species, and the percentage of polymorphic bands was 100%. The percentage of polymorphic loci within species ranged from 3.61 to 58.56%. Chaeturichthys stigmatias showed the greatest percentage of polymorphic loci (58.56%), the highest Nei’s genetic diversity (0.2421 ± 0.2190) and Shannon’s information index (0.3506 ± 0.3092), while Pterogobius zacalles showed the lowest percentage polymorphic loci (3.61%), the lowest Nei’s genetic diversity (0.0150 ± 0.0778) and lowest Shannon’s information index (0.0219 ± 0.1136). The topology of UPGMA tree showed that the individuals from the same species clustered together and the 10 species formed two major clades. One clade consisted Cryptocentrus filifer, P. zacalles, Tridentiger trigonocephalus,Chaeturichthys hexanema, C. stigmatias, Acanthogobius flavimanus andSynechogobius ommaturus, and the other clade consisted Odontamblyopus rubicundus, Trypauchen vagina and Ctenotrypauchen microcephalus. The results agreed with the traditional taxonomy of the morphological characters. AFLP fingerprints were successfully used to study the phylogenetic relationship of the gobioid fishes and it identified species origins of morphologically similar taxa. Key words: Phylogenetic, amplified fragment length polymorphism (AFLP), gobiidae, Amblyopinae, gobiinae.
Highlights
In recent studies, molecular markers have been proved to be effective tools to estimate phylogenetic relationships at various taxonomic levels (Miya and Nishida, 1996, 2000; Forey et al, 1996)
The values of observed number of alleles were from 1.0361 ± 0.1878 (P. zacalles) to 1.5856 ± 0.4949 (C. stigmatias) and effective number of alleles was from 1.0256 ± 0.1328 (P. zacalles) to 1.4386 ± 0.4207 (C. stigmatias) (Table 3)
Assuming Hardy-Weinberg equilibrium, species C. stigmatias showed the greatest percent polymorphic loci (58.56%), the highest gene diversity (0.2421 ± 0.2190) and Shannon’s diversity index (0.3506 ± 0.3092), while species P. zacalles showed the lowest percentage of polymorphic loci (3.61%), the lowest gene diversity (0.0150 ± 0.0778) and Shannon’s diversity index (0.0219 ± 0.1136)
Summary
Molecular markers have been proved to be effective tools to estimate phylogenetic relationships at various taxonomic levels (Miya and Nishida, 1996, 2000; Forey et al, 1996). Among different types of molecular markers, amplified fragment length polymorphism (AFLP) analysis is a PCR-based, multi-locus fingerprinting technique that combines the strengths and overcomes the weaknesses of the RFLP and RAPD methods (Vos et al, 1995), and it is highly efficient for the detection of genetic variations. It allows fast and efficient generation of a large amount of genetic data.
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