Abstract
This work was done to study the bacterial diversity of mithun (Bos frontalis) fed on mixed tree leaves and rice straw based diet. Genomic DNA was extracted from the rumen liquor of mithun, 16S rDNA sequences were amplified, cloned and randomly selected for sequencing. The nearest neighbors were retrieved from the NCBI through a BLAST search and a phylogenetic tree was constructed. In our findings, 12% of clones showed similarity with known bacterial species (Prevotella ruminicola, Butyrivibrio fibrisolvens, Pseudobutyrivibrio ruminis, Succinivibrio dextrinosolvens and Ruminococcus flavefaciens) and 6% of clones showed similarity with known bacterial genus (Butyrivibrio species, Streptococcus species) of 97-100% similarities. Twenty-two percent of clones showed similarity with known bacteria (P. ruminicola, Prevotella species, Sporanaerobacter acetigenes, Clostridiales bacterium, Bacteroidetes bacterium) of 90-97% similarities. Sequences of all the clones were also classified by using taxonomic classifier software available at Ribosomal Database Project and classification showed that, all the clones were under four phyla, namely Bacteroidetes (54%), Firmicutes (36%), Proteobacteria (4%) and Tenericutes (2%). The experiment showed that, bacterial population in the rumen of mithun fed on mixed tree leaves and rice straw based diet harbor diversified species of bacteria responsible for lignocellulosic feedstuffs. Key words: Tree leaves, bacteria, clones, sequences.
Highlights
The North-East India, being at the confluence of three major bio-geographical realms of the world, is extremely rich in floral and faunal biodiversity with several endemic species
50% of dry matter (DM) requirement was met through concentrate mixture and rest through mixed tree leaves and paddy straw (2:1 ratio on fresh basis) according to the standard developed in the institute
The crude protein content of tree leaves/shrubs reported in this experiment is comparatively higher than the green fodder of other parts of
Summary
The North-East India, being at the confluence of three major bio-geographical realms of the world, is extremely rich in floral and faunal biodiversity with several endemic species. Mithun (Bos frontalis) is considered as the most important bovine species and the people use them as pride object of social sacrifice but as life currency in their local transactions (Annual Report, 2012) This unique bovine species is believed to be domesticated more than 8000 years ago and is mainly available in the four north-eastern hilly states of Arunachal Pradesh, Nagaland, Mizoram and Manipur. Farmers do not provide any additional supplement except for occasional common salt feeding, especially at the time of restraining for some purposes (Moyong, 2012) The performance of this species of animal was found to be satisfactory in confinement when reared on tree leaves based ration (Das et al, 2010). The rumen bacterial diversity of mithun were studied, fed on mixed tree leaves and rice straw based diet by amplification, cloning and sequencing of 16S rRNA gene of bacteria, followed by sequence comparison and phylogenetic analysis
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
