Abstract
The amplification and sequencing of 16S rDNA are useful tools to identify important bacteria that cause diseases in the respiratory tracks of a variety of domestic animals. Bordetella bronchiseptica, one of such bacterium, has demonstrated some changes in its metabolic core, generating a greater need for identification through amplification and sequencing of 16S rDNA. We applied this methodology to eleven nasal and pharyngeal isolates from dogs to detect B. bronchiseptica. Eight strains were completed from which seven showed 98-99% homology; and three strains were only partially sequenced. All eleven strains sequences were deposited in the GenBank (NCBI). What we found was that the sequencing of B. bronchiseptica 16S rDNA is an excellent tool that certifies conventional methods of identification and avoids environmental challenges. Key words: Bordetella bronchiseptica, 16S rDNA, canine bacteria.
Highlights
The id dentific cation of Bordetellla bron nchise eptica ffrom canin ne isollates of o sequ uencing 16S riboso omal D
The amplification and sequencing s of o 16S rDNA are useful to ools to identtify importan nt bacteria th hat cause diseases in n the respira atory tracks of a variety of domestic c animals
The two reference strains from the American Type Culture Collection (ATCC) were 4617 and 10580
Summary
The id dentific cation of Bordetellla bron nchise eptica ffrom canin ne isollates of o sequ uencing 16S riboso omal D. The amplification and sequencing s of o 16S rDNA are useful to ools to identtify importan nt bacteria th hat cause diseases in n the respira atory tracks of a variety of domestic c animals. B. Bordetella brronchiseptica a, one of such bacterium, has de emonstrated d some changes in its m metabolic corre, generatin ng a greater need for identificatio on through amplification n and seque encing of 1 6S rDNA. W. We applied tthis methodology to eleven nas sal and pharryngeal isolates from dogs d to dete ect B. bron nchiseptica. All eleven strains sequ that the se equencing of B. bronch hiseptica 16S
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