Abstract
Eight Random Amplified Microsatellite markers (RAMs) were used to characterize the genetic diversity found in 14 Prunus materials belonging to the deciduous collection of the Pedagogical and Technological University of Colombia. A total of 121 bands were generated: they range from nine for the GT primer to 26 for the ACA primer, and have molecular weights between 100 and 2050 Kb. At a Nei-Li similarity level of 0.75, four groups were formed, according to the characteristics of the fruit. The number of polymorphic loci varied between 8 (GT and AG) and 21 (ACA); the higher levels of heterozygosity were CA (0.43) and CT (0.41). The average value of heterozigosity for the total population was 0.35, much lower than those found in other Prunus species, but higher than other fruit species where RAM markers were used. Therefore, strategies must be deployed for the collections in order to increase genetic variability, such as the introduction of wild or hybrid materials. The RAM technique proved useful as a method for assessing genetic diversity in species of the Prunus genus. Key words: Prunus domestica, genetic diversity, Random Amplified Microsatellite markers (RAMs), deciduous.
Highlights
In the last decades of the twentieth century, fruit market has been on the increase, so one can say the century is exotic fruits century, which are mostly of tropical origin.Colombia is a mega diverse country with great potential to increase fruit production
Among the Simple Sequence Repeat markers, we find that Random Amplified Microsatellite (RAMs) are very useful for measuring genetic diversity in plants and animals, difference between families, between and within species (Muñoz et al, 2008), show the basis of variation of individuals, allow you to select specific regions within the DNA molecule for determined studies, the number of polymorphisms detectable is theoretically unlimited and it is possible to analyze both information that is expressed (Henríquez, 2000)
This research aims to identify the genetic variability present in the collection of plum (Prunus domestica) of the University Pedagogical and Technology of Colombia, to establish genetic relationship that exists between the materials and thereby provide a tool that can be used as breeding strategies on the species and to identify elite material that may be a new productive alternative for our farmers
Summary
In the last decades of the twentieth century, fruit market has been on the increase, so one can say the century is exotic fruits century, which are mostly of tropical origin. Among the Simple Sequence Repeat markers, we find that Random Amplified Microsatellite (RAMs) are very useful for measuring genetic diversity in plants and animals, difference between families, between and within species (Muñoz et al, 2008), show the basis of variation of individuals, allow you to select specific regions within the DNA molecule for determined studies, the number of polymorphisms detectable is theoretically unlimited and it is possible to analyze both information that is expressed (Henríquez, 2000) This methodology is feasible for small laboratories in terms of equipment and facilities cost, does not require prior knowledge of sequences and the use of radioactive isotopes (Hantula et al, 1996). This research aims to identify the genetic variability present in the collection of plum (Prunus domestica) of the University Pedagogical and Technology of Colombia, to establish genetic relationship that exists between the materials and thereby provide a tool that can be used as breeding strategies on the species and to identify elite material that may be a new productive alternative for our farmers
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