Abstract
Two hundred and fifty different samples were collected from bovine and examined for the presence of staphylococcal bacteria. 189 isolates were able to grow on the mannitol salt agar (MSA), known as staphylococci. Coagulase test revealed that 165 isolates were able to produce this enzyme; 138 of these isolates were Staphylococcus aureus which appeared in 55.2% of the isolates. Deoxyribonuclase (DNAase), urase and beta haemolysis activities of the isolates were also investigated and it showed 90.69, 86.23, and 87.86% of the isolates respectively. An enzymatic examination of the isolates was combined in numerous tests like catalase test, coagulase test, non- producing oxidase, sugar fermentation, oxidative and fermentation test, liquefaction of gelatin and MR-VP test. The polymerase chain reaction (PCR) amplification of coa gene products of S. aureus showed the following: gene product of 500 bp (22.5%); 650 bp (15%); 800 and 850 bp (25% for each); and 600 bp (12.5%). Key words: Staphylococcus aureus, bovine, coagulase, coa gene, polymerase chain reaction (PCR).
Highlights
Staphylococcus aureus can infect any part of the body; it causes some diseases in humans and animals, ranging from skin infection, food poisoning, brain abscesses and outbreak in post operative wound infection (Kenneth, 2008)
The results showed that 189 out of 250 bovine samples
S. aureus isolates were similar in some biochemical tests like catalase, oxidase, coagulase, O/F, ONPG, VP, MR, sugar fermentation, gelatin liquefaction, latex agglutination and API Staph
Summary
Staphylococcus aureus can infect any part of the body; it causes some diseases in humans and animals, ranging from skin infection, food poisoning, brain abscesses and outbreak in post operative wound infection (Kenneth, 2008). The analysis of coagulase encoding S. aureus DNA coa gene has demonstrated variable sequences in the 3 ́ end coding region (Goh et al, 1992). This region contains a polymorphism repeat region that can be used to differentiate S. aureus isolates.
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