Abstract
This study was conducted in five regional states of Ethiopia from January 2011 to March 2012 with the objective of identifying the serotypes of foot and mouth disease by molecular technique in Ethiopia. Epithelial tissue samples were collected from cattle and swine found in the foot and mouth disease outbreak areas of the country and submitted to the National Veterinary Institute, DebreZeit, Ethiopia and World Reference Laboratory for Foot and Mouth Disease, Pirbright, UK. Thus, virus isolation and serotype identification were performed. From a total of 59 samples, cytopathic effect was observed in 43 (72.88%) samples in BHK-21 cell culture. Serotyping of foot and mouth disease viruses were done by applying agarose gel-based RT-PCR at the National Veterinary Institute, and by cell culture ELISA at World Reference Laboratory for Foot and Mouth Disease. Serotype O was recorded throughout the country where outbreaks occurred. Regular investigation of foot and mouth disease outbreaks is important to have more detailed information on the serotypes and topotypes circulating in Ethiopia and for effective vaccine development. Key words: Ethiopia, foot and mouth disease (FMD), serotype.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
