Abstract
During the 2012 growing season, thirty three samples were collected from squash, pumpkin and muskmelon plants showing virus-like symptoms in Riyadh and Al-Madena regions of Saudi Arabia. Eleven of these samples were found positive for Zucchini yellow mosaic virus (ZYMV) by double antibody sandwich ELISA (DAS-ELISA). In the host range study for the five selected ZYMV isolates, 11 out of the 22 mechanically inoculated test plants were infected and showed variable symptoms. The amplification of viral DNA product through reverse transcription-polymerase chain reaction amplification (RT-PCR) using a primer pair specific for ZYMV, yielded fragments of approximately 1185 bp. Southern blot hybridization confirmed the results obtained through RT-PCR, using a specific DNA probe homologous to ZYMV. Nucleotide sequences for the coat protein gene from all five Saudi isolates of ZYMV indicated a similarity of 97.1-100.0% between them. Comparative analysis of the nucleotide sequences of coat protein gene from the Saudi isolates and other ZYMV isolates obtained from NCBI, showed a relatively high nucleotide sequence similarity that ranged between 92.0-98.8%. The highest similarity was found with Syria, Jordan, Iran, Hungarian, Austria, Slovenia and Germany isolates (97.1 to 98.8%). The nucleotide sequence data obtained for the five ZYMV isolates was deposited in the GenBank under the accession numbers JQ899263, JQ899264, JQ899265, JQ899266 and JQ899267. Key words: ZYMV, DAS-ELISA, RT-PCR, Hybridization, Sequence.
Highlights
Characterizattion of differe ent iso olates o of Zuc cchini y yellow w mosaic m f cucurb c bits in S
Southern blot hybridization con nfirmed the results obta ained through reverse transcriptiion-polymera ase chain reaction amplificatio on (RT-PCR), u using a spec cific DNA
Zuccchini yellow w mosaic viirus (ZYMV) is a species belo onging to the e genus Potyv virus in the family Potyvirid dae and d characteriz zed by a monopartite, m positive-sens se, sing gle-stranded RNA genom me encapsidatted in flexuou us, entous particles
Summary
Eleven of these t samples were found positive e for Zucch hini yellow m mosaic virus s (ZYMV) by y double antibody sa andwich ELIS. In the host range s study for the e five selecte ed ZYMV iso olates, 11 out of the 22. Southern blot hybridization con nfirmed the results obta ained through RT-PCR, u using a spec cific DNA probe homo ologous to ZYMV. Nucleo otide sequen nces for the coat protein gene from a all five Saudii isolates of ZYMV in ndicated a similarity of 97.1-100.0%. MV isolates obtained fro om NCBI, showed a relatively r hig gh nucleotide sequence similarity th hat ranged b between 92.0. The nucleottide sequenc ce data obta ained for th he five ZYMV
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