Abstract
Fusarium crown and root rot of tomato (Solanum lycopersicum) is the disease caused by the fungal pathogen Fusarium oxysporum f.sp. radicis-lycopersici(FORL). The most effective way to control this disease is to plant resistant varieties. Markers tightly linked to Fusarium crown and root rot could be used in breeding programs to introgress crown rot resistance into new varieties. In this study, we converted the random amplified polymorphic DNA (RAPD) marker UBC#116, linked to the Fusarium crown and root rot resistance gene (Frl), into a co-dominant sequence characterized amplified region (SCAR) marker. A fragment of about 480 bp, linked to the Frl gene, was polymerase chain reaction (PCR) amplified with the use of the UBC#116 primers, cloned and sequenced. A pair of primers were designed and PCR amplification was performed to develop a new SCAR marker for the Frl gene. The new marker was applied for the analysis of 96 tomato genotypes. The RAPD marker UBC#116 was also used and it revealed that the markers were equivalent to each other. However, the development of the new co-dominant SCAR marker has made marker-assisted selection (MAS) more practical, rapid and efficient. Key words: Fusarium oxysporum f. sp. radicis-lycopersicum (FORL), marker-assisted selection (MAS), Solanum lycopersicum, breeding.
Highlights
The soil-borne fungus, Fusarium oxysporum f.sp. radicislycopersici (FORL), which causes Fusarium crown and root rot of tomato (Solanum lycopersicum), was first observed in the southern part of Japan in 1965
Three random amplified polymorphic DNA (RAPD) markers, UBC#116, 194, 655, linked to Frl (Fazio et al 1999), were initially screened on 96 tomato lines collected from NIHHS, Rural Development Administration (RDA), Korea (Figure 1)
polymerase chain reaction (PCR) based methodology would be more suitable than restriction fragment length polymorphism (RFLP) technique because of its lower cost and ease of use
Summary
The soil-borne fungus, Fusarium oxysporum f.sp. radicislycopersici (FORL), which causes Fusarium crown and root rot of tomato (Solanum lycopersicum), was first observed in the southern part of Japan in 1965. Frl is linked with both tobacco mosaic virus resistant genes, Tm-1 (Elkind, 1988) and Tm-2 (Laterrot and Couteaudier, 1989), which are alleles at the same locus. Several studies have reported that the Frl gene is tightly linked with restriction fragment length polymorphism (RFLP) marker TG101, which is linked to Tm-22, on chromosome 9 (Young et al, 1988; Young and Tanksley, 1989; Laterrot and Moretti, 1991). Markers tightly linked to the Frl gene could be used as breeding tools for the introgression of crown rot resistance into new varieties to avoid the inconsistent virulence of FORL caused by the influence of environmental factors on disease development (Jones et al, 1990). The efficiency of selection and flexibility of a breeding program will be increased
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