Abstract
The over exploitation of African mahogany in tropical forest has threatened the genetic base of this useful timber and medicinal tree species and as such, an experiment was conducted on the in vitro culture of Khaya grandifoliola, an endangered tree species commonly found in the high forest zones of West Africa to explore its potential for micropropagation. Embryos excised from matured seeds were cultured on Murashige and Skoog (MS) medium supplemented with naphthalene acetic acid (NAA), benzyl amino purine (BAP) and kinetine (KIN) at different concentrations. The optimum result in relation to shoot length, root length, number of nodes and number of root was obtained on MS medium supplemented with 1.0 mg/L BAP + 0.01 mg/L NAA. Key words: Khaya grandifoliola, African mahogany, in vitro culture, medium, embryo, plantlets.
Highlights
The sub-Saharan Africa is endowed with tropical forest rich in valuable timber tree species which are of immense benefit to the local people in meeting both their food and medicinal needs
The over exploitation of African mahogany in tropical forest has threatened the genetic base of this useful timber and medicinal tree species and as such, an experiment was conducted on the in vitro culture of Khaya grandifoliola, an endangered tree species commonly found in the high forest zones of West Africa to explore its potential for micropropagation
Embryos excised from matured seeds were cultured on Murashige and Skoog (MS) medium supplemented with naphthalene acetic acid (NAA), benzyl amino purine (BAP) and kinetine (KIN) at different concentrations
Summary
The sub-Saharan Africa is endowed with tropical forest rich in valuable timber tree species which are of immense benefit to the local people in meeting both their food and medicinal needs. Most of the K. grandifoliola exists in the wild state; the regeneration and long term conservation of these species are at the mercy of the vagaries of nature and the profit driven herb collectors and timber merchants. Plant regeneration from tissues cultured in vitro has been accomplished in a range of forest species (Thorpe et al, 1991), there is no. 1.00 1.00 1.00 1.00 3.00 1.00 3.00 1.00 2.00 0.00 report on successful in vitro plant regeneration of K. grandifoliola. Considering the fact that this forest tree species seeds are recalcitrant in nature and producing adequate number of seedlings for any meaningful plantation establishment programme from seeds stored for long time is very difficult, this present work aims to describe a reliable plant regeneration protocol from matured seed embryo
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