Abstract
Sequence alignment of the 897 bp amplicon obtained from a diseased sunn hemp (Crotalaria juncea L.) plant DNA revealed a complete 771 bp coat protein (CP) gene flanked by 3’ regions of the AV2 and AC3 genes. Southern hybridization using (α-32P) dCTP labeled (CP) gene probe of Indian tomato leaf curl virus (IToLCV) demonstrated the association of begomovirus with the leaf curl disease of sunn hemp. Phylogenetic data suggested that, the AV2, CP and AC3 genes have closest genetic relationship with begomovirus isolates from India, China and Bangladesh, respectively. In silico recombination analysis elucidated a 297 nucleotides hot spot (346 to 643 nucleotides) within AV2 overlapping region of CP gene, amenable to genetic rearrangements, with lineage from tomato leaf curl virus Bangalore (ToLCuVB) and Indian cassava mosaic virus-Ind (ICMV) as major and minor parents, respectively. Thus, it is concluded that the recombinant CP genes related to begomoviruses are evolved from the Indian isolates, causing broad host specificity and molecular diversity among the related begomoviruses across the geographical limits of Southeast Asia. Key words: Begomovirus, sunn hemp, coat protein, recombination, phylogenetic analysis, in silico analysis.
Highlights
Sunn hemp (Crotalaria juncea L.) is a sub-tropical annual legume that belongs to the family of Fabaceae
The genus Begomovirus typically have bipartite, circular single-stranded DNA genomes with all functions required for virus replication, control of gene expression and encapsidation encoded on DNA-A and genes involved in intra and inter-cellular movement encoded on DNA-B (Bowdoin et al, 1999)
Earlier investigations based on polymerase chain reaction (PCR) and hybridization studies have indicated the associations of begomovirus with leaf curl disease in sunn hemp plants (Khan et al, 2002; Raj et al, 2003)
Summary
Sunn hemp (Crotalaria juncea L.) is a sub-tropical annual legume that belongs to the family of Fabaceae. Sequence alignment of the 897 bp amplicon obtained from a diseased sunn hemp (Crotalaria juncea L.) plant DNA revealed a complete 771 bp coat protein (CP) gene flanked by 3’ regions of the AV2 and AC3 genes.
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