Abstract
The major limitation in large-scale cultivation of Jatropha curcas for use as energy crop is the inconsistent and unstable seed yield due to the heterozygous nature of the plant. A reliable in vitro regeneration system is necessary for continuous supply of quality planting material at large-scale. In this study, the interaction between the season collection of explants and capacity of in vitro regeneration shoots from foliar explants was investigated. Three genotypes selected in our breeding program were evaluated. We achieved an average of 39.8, 25.5 and 10.9 shoots per explant for G1, G2 and G3 genotypes, respectively. All genotypes showed higher regeneration capacity when the foliar explants were collected in September/2012 season. Excellent results were obtained with the use of micrografting technique for the in vitro rooting, with a plant recovery rate of 85%. In order to confirm the genetic stability of micropropagated shoots, two analyses were performed: ploidy estimation using flow cytometry and DNA polymorphism analysis using TRAP molecular markers, which has been here reported for the first time for J. curcas. For G1 genotype, it was found that 4% of the plants were tetraploid and 5% of plants had polymorphic bands. No DNA polymorphisms were found in plants of other genotypes. Thus, the low or no somaclonal variation indicates that the protocol established preserves the clonal fidelity of micropropagated plants. Key words: Organogenesis, in vitro micrografting, foliar explants.
Highlights
Mic cropro opagation of Jatrop pha cu urcas s superio or genottypes and a ev valuatio on of c clonal fidelity y by ta arget region n ampliificatio on poly ymorphism ((TRAP)) mole ecular mark ker and d flow c cytometry
This paper reports efficient micropropagation protocols via organogenesis using foliar explants for the cloning of three J. curcas genotypes selected in our breeding program due to its superior oil yield and quality
The season collection of foliar explants into matrix plants had a great influence in the shoot regeneration of the three studied genotypes
Summary
Mic cropro opagation of Jatrop pha cu urcas s superio or genottypes and a ev valuatio on of c clonal fidelity y by ta arget region n ampliificatio on poly ymorphism ((TRAP)) mole ecular mark ker and d flow c cytometry. The major limitation in large-scale cultivatio on of Jatrop pha curcas for use as energy cro op is the inconsisten nt and unsta able seed yie eld due to the t heterozy ygous nature e of the plan nt. A reliable e in vitro regeneratio on system is s necessary for continuo ous supply o of quality planting mate erial at large--scale. In this study,, the intera action betwe een the sea ason collecttion of exp plants and c capacity of in vitro regeneratio on shoots fro om foliar ex xplants was investigated d. P molecular markers, w which has been here reported fo or the first time t for J. curcas
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