Abstract
PCR-based methods were frequently used for identification and analysis of microorganisms. In this study 8 Streptococcus strains isolated from Giant Pandas in different parts of China were identified by amplification of 16S ribosomal RNA sequence by PCR. Genomic diversities of 8 identified Streptococcus strains were analyzed by repetitive extragenic palindromic PCR (REP-PCR) and enterobacterial repetitive intergenic consensus sequence PCR (ERIC-PCR). REP-PCR and ERIC-PCR fingerprinting showed different patterns of each Streptococcus. The cluster analysis revealed there is a low level of similarity among these isolated Streptococci which were separated into different subtypes and the same serotype Streptococci were divided into sub-genotypes. REP-PCR and ERIC-PCR fingerprinting results were correlated with serotype. The results also demonstrated the two PCR methods have good discriminative ability and have potential as rapid methods for typing Streptococcus as well as high genomic diversity of Streptococci of pandas. Keywords: Streptococcus, REP-PCR, ERIC-PCR, serotype, Giant Panda.
Highlights
The Giant panda, Ailuropoda melanoleuca, is one of the most rare species in the world and are unique to China, they are the First-grade state protected animal in China, who are praised as "Living Fossil" and "National Treasure" of our country
Genomic diversities of 8 identified Streptococcus strains were analyzed by repetitive extragenic palindromic PCR (REP-PCR) and enterobacterial repetitive intergenic consensus sequence PCR (ERIC-PCR)
The results demonstrated the two PCR methods have good discriminative ability and have potential as rapid methods for typing Streptococcus as well as high genomic diversity of Streptococci of pandas
Summary
The Giant panda, Ailuropoda melanoleuca, is one of the most rare species in the world and are unique to China, they are the First-grade state protected animal in China, who are praised as "Living Fossil" and "National Treasure" of our country. Streptococcus species are widely distributed in nature, such as water, dust, alimentary tract, respiratory and genitourinary tracts, milk, etc. They are major pathogens for animals and human beings. There are different typing methods for Streptococcus spp. The traditional classification is based on hemolytic or antigenic characteristics. With the development of molecular biology, methods based on PCR technique had been recognized as having the great discriminative ability to differentiate isolated strains by
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