Abstract
The production of gallic acid in cell suspension culture of Acer ginnala Maxim wasstudied. Some physiochemical factors and chemical substances effect on the cell growth and the production of gallic acid were investigated. Cells harvested from plant tissue culture were extracted and applied to high performance liquid chromatography to measure gallic acid content. 0.008 mg.L-1 TDZ and 0.1 mg.L-1BA was optimal for the cell growth. 0.004 mg.L-1 TDZ and 0.1 mg.L-1 BA was best for the production of gallic acid. Maintaining the initial pH value at 5.8 was most suitable for gallic acid accumulation in A. ginnala Maxim cell suspension cultures. To satisfy the condition of mass-producing gallic acid in the suspension culture, the adapted inoculum quantity was 30 g.L-1. The results also provided evidence that the optional culture period was 7 days with light. Key words: Acer ginnala Maxim, gallic acid, cell growth, suspension culture, physiochemical factor.
Highlights
Acer ginnala Maxim (Amur Maple) is a species of maple native to northeastern Asia from easternmost Mongolia east to Korea and Japan, and north to southeastern Siberia in the Amur River valley
Cells harvested from plant tissue culture were extracted and applied to high performance liquid chromatography to measure gallic acid content. 0.008 mg.L-1 TDZ and 0.1 mg.L-1 BA was optimal for the cell growth. 0.004 mg.L-1 TDZ and 0.1 mg.L-1 BA was best for the production of gallic acid
The exogenous hormones’ categories, concentration and proportion could influence on cell biomass and metabolites content in plant cell suspension culture (Dai and Li, 2007)
Summary
Acer ginnala Maxim (Amur Maple) is a species of maple native to northeastern Asia from easternmost Mongolia east to Korea and Japan, and north to southeastern Siberia in the Amur River valley. It is a deciduous spreading shrub or small tree growing to 3 to 10 m tall, with a short trunk up to 20 to 40 cm diameter and slender branches. The leaves turn brilliant orange to red in autumn, and are on slender, often pink-tinged, petioles 3 to 5 cm long (Rushforth, 1999). The young leaves were used as a tea substitute (Kunkel, 1984). High yields of polyphenol were obtained from A. ginnala Maxim (Carr, 1985)
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