Abstract
Trichomoniasis is a sexually transmitted disease caused by a mobile flagellate pathogenic protist protozoan of the urogenital tract, Trichomonas vaginalis. No data is available in Cote d’Ivoire about its diagnosis by molecular methods. This study aims to identify T. vaginalis by polymerase chain reaction (PCR) among women at Institut Pasteur of Cote d’Ivoire. Vaginal swabs were obtained from each woman from July to October, 2013. For T. vaginalis detection, Giemsa stain for microscopic examination and real-time PCR were performed. The PCR targeted 67 bp region of a repeated sequence of the T. vaginalis genome. A positive T. vaginalis result was defined as a cycle threshold (Ct) less than 36. The results show that of the 194 specimens tested by both Giemsa stain method and Real-time PCR, 2 were positive to Giemsa stain (1.03%) and 7 were positive in PCR assay (3.61%). Women who had multiple sexual partners in the last two months were found to be the most infected. Comparing the two methods, real-time PCR had a higher sensitivity (100%) and specificity (97.40%). This study shows that the prevalence rate of T. vaginalis infection remains low. However, using the PCR approach allows for better detecting infection than conventional staining method. Key words: Trichomonas vaginalis, real-time polymerase chain reaction (PCR), Giemsa stain, Cote d’Ivoire.
Highlights
Trichomoniasis is a sexually transmitted disease caused by a mobile flagellate pathogenic protist protozoan of the urogenital tract, Trichomonas vaginalis
The results show that of the 194 specimens tested by both Giemsa stain method and Real-time polymerase chain reaction (PCR), 2 were positive to Giemsa stain (1.03%) and 7 were positive in PCR assay (3.61%)
This study shows that the prevalence rate of T. vaginalis infection remains low
Summary
Trichomoniasis is a sexually transmitted disease caused by a mobile flagellate pathogenic protist protozoan of the urogenital tract, Trichomonas vaginalis (Workowski et al, 2015). This infection is one of the most common non-viral sexually transmitted diseases such as chlamydia, gonorrhea, and syphilis (WHO, 2015). The prevalence rates of T. vaginalis in women population varies from 5 to 74% according to the methods of diagnosis (Johnston and Mabey, 2008; Korycińska et al, 2017). No data about molecular diagnostic of T. vaginalis infection is available in Côte d’Ivoire; the aim of this study is to identify T. vaginalis by PCR and associated factors among women at Pasteur Institute of Côte d’Ivoire
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