Abstract
Asclepiadaceae is an economically important family species which are the source of fiber, rubber and dyes. In this study, genetic variability has been determined in three species, that is, Tylophora hirsuta, Wattakaka volubilis and Cryptolepis buchananii. The aim of present study was to understand the extent and pattern of genetic diversity among the individuals of same and different species of Asclepiadaceae. To asses the level of polymorphism within the species and members of different species, randomly amplified polymorphic DNA (RAPD) markers were used. Sixty RAPD primers of OPA, OPC, OPF and OPG series were used; only eight primers of OPC series gave amplification. Maximum polymorphism at interspecific and intraspecific levels was shown by OPC 09 and minimum polymorphism was observed in OPC 05. The data was analyzed using software numerical taxonomy system (NTSYS) cluster analysis PC version 2.20. In total 190 monomorphic and 78 polymorphic bands were produced from all primers. Therefore, out of 322 amplified products, 59% were monomorphic and 24.22% were polymorphic. Low genetic diversification was found both at intraspecific and interspecific level. Mixed pattern of grouping in the analyses indicated the close affinities of species with each other. Key words: Comparative assessment, genetic diversity, Asclepiadaceous species, randomly amplified polymorphic DNA (RAPD) markers on Asclepiadaceae.
Highlights
Asclepiadaceae have 175 to 180 genera and 2000 species which are distributed mainly in the tropical and subtropical regions of the world
Isolated genomic DNA was run on 1% agarose gel after treatment with RNase A for determining the quality and quantity of DNA
In polymerase chain reaction (PCR), high quality of DNA is required for amplification purpose; good quality DNA was confirmed by agarose gel electrophoresis
Summary
Asclepiadaceae have 175 to 180 genera and 2000 species which are distributed mainly in the tropical and subtropical regions of the world. In Pakistan, they are distributed in North Punjab, Azad Kashmir, Hazara, Rawalpindi, Attock and salt range (Nasir and Ali, 1983). Members of this family are mostly herbs, lianas, shrubs or trees (rarely) which are laticiferous. Molecular markers have been looked upon as tools for a large number of applications ranging from localization of a gene to improvement of plant varieties by markerassisted selection. They have become extremely popular markers for phylogenetic analysis adding new dimensions to the evolutionary theories (Joshi et al., 1999). Volubilis, using RAPD markers in order to establish polygenetic relationship among these genera
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