Abstract
Tuberculosis is a serious infectious disease caused by Mycobacterium tuberculosis. DNA vaccination is an advanced technique for protecting human bodies from infectious diseases including tuberculosis by injecting exogenous gene engineering DNA into the body to produce an immunological response. In this study, we examined the immunogenicity and protective efficacy of DNA vaccine (pCDNA-MPB64) expressing MPB64 protein and its booster effects in mice for controlling tuberculosis. The results showed that MPB64 DNA vaccine led to a dramatic augmentation of humoral and cellular responses. All these suggested that MPB64 DNA vaccines is an ideal vaccine and may be further developed as a useful method to prevent tuberculosis. Key words: Tuberculosis, DNA vaccines, MPB64, recombination plasmid, antibody titer.
Highlights
Tuberculosis is a common, serious and sometimes lethal infectious disease caused by Mycobacterium tuberculosis
The results showed that MPB64 DNA vaccine led to a dramatic augmentation of humoral and cellular responses
The gene of MPB64 was first amplified by PCR and M. tuberculosis H37Rv genomic DNA as the template
Summary
Tuberculosis is a common, serious and sometimes lethal infectious disease caused by Mycobacterium tuberculosis. The most common vaccine against tuberculosis currently is Bacillus Calmette-Guérin (BCG), prepared from a strain of the attenuated or weakened live bovine tuberculosis bacillus. BCG could interfer the test of purified protein derivative (PPD, known as the Mantoux screening test), a diagnostic tool for tuberculosis, making the PPD test very confused (Kernodle et al, 2010). It makes the routine quarantine more difficult after vaccinated with BCG since natural infection and artificial immunity would be undistinguished. It is very important to discover the new vaccine for the diagnosis and prevention of the tuberculosis
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