Abstract
Leuzea (Rhaponticum carthamoides) is a valuable medicinal plant from Asteraceae. Micropropagation could be a good alternative for the mass propagation of Leuzea carthamoides. To investigate the callogenesis of leaf explants, 12 different hormonal combinations including different concentrations of 16-benzylaminopurine (BA) and 2,4-dichlorophenoxyacetic acid (2, 4-D) were studied in two separable experiments. In both experiments, the explants were transferred to the Ms medium supplemented with 0.5 mg L-1 indole acetic acid (IAA) and 0.5 mg L-1 BA for 7 and 50 days after culture for regeneration, respectively. Then, after one month the percentages of callogenesis and the amount of produced callus were measured. In other experiment to investigated regeneration of root explants, 9 different hormonal combinations were studied including different concentrations of BA and IAA. The number of leaf per explants, length of greatest leaf per explant and regeneration percentage were measured one month after culture. The maximum callus production was obtained using 1 mg L-1 2, 4-D and 1.5 mg L-1 BA and 0.25 mg L-1 2, 4-D and 1.5 mg L-1 BA in first experiment and second experiment, respectively. In the third experiment, root explants had direct regeneration and medium with 0.5 mg L-1 BA + 1 mg L-1 IAA can be suitable medium. Key words: Leuzea carthamoides, in vitro, regeneration, callus induction.
Highlights
Leuzea (Rhaponticum carthamoides) is a valuable medicinal plant from the family Asteraceae (Orlova et al, 2000)
The present study aims to determine the effect of different concentrations of (BA) and (2,4-D) on callus induction of L. carthamoides through the culture of leaf explants and investigate the effect of different concentrations of BA and indole acetic acid (IAA) on regeneration of root explants
In summary, present experiment showed that use of leaf and root explants for micropropagation is beneficial
Summary
Leuzea (Rhaponticum carthamoides) is a valuable medicinal plant from the family Asteraceae (Orlova et al, 2000). R. carthamoides is a perennial herb, commonly known as a maral root or Russian leuzea, which has been used for centuries in Eastern parts of Russia due to its marked medicinal properties (Kokoska and Janovska, 2009). The West and East of Siberia, Northern Mongolia and central Asia are its natural habitats. It is a medicinal herb with a tonic effect (Selepcova et al, 1993). 20- hydroxy-Ecdison or Leuzine is the most important compound present in ecdysterone (Omidbaigi, 2007). RCE improves sleep, appetite, moods, mental and physical state, and functional ability of humans under working conditions (Yance, 2004)
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