Abstract
Antioxidant properties of plants can help thwart a plethora of conditions including cancer, bronchitis, asthma, ulcers, coronary heart diseases, scabies, insomnia, senile debility, diabetes, dysentery, etc. In this study, Andrographis paniculata (Family: Acanthaceae) was investigated for antioxidant activity. 1, 1-Diphenyl-2-picryl hydrazyl (DPPH), reducing power assay, total antioxidant capacity, 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), nitric oxide test, total phenolic and flavonoid from methanol, acetone, and water extracts were investigated employing various established in-vitro system. The results divulged that methanol extract had significant activity in quenching of DPPH, reducing power, ABTS, nitric oxide, antioxidant capacity when compared to other extracts. All extracts were correlated with two specific standard antioxidants ascorbic acid and butylated hydroxyanisole. The methanol extract at 500 µg/ml showed the highest scavenging activity of DPPH (91.78 ± 0.02%), ABTS (99.76 ± 0.05%). Total antioxidant capacity was 83.35 ± 0.13 AAE/mg. Reducing the power of methanol extract was significant compared to all other fractions. Nitric oxide scavenging activity was maximum in acetone extract (80.73 ± 0.05%). The quantitative estimation of the extract revealed a considerable amount of flavonoids and conversely, the amount of phenolic compounds was poor. The flavonoid content (592.34 ± 0.13 mg QE/g of dry extract) was maximum in methanol extract but phenolic content of methanol and acetone extract was very similar. This study strongly suggests that methanol extract exhibits more potent antioxidant activity than other extracts of acetone and water. Key words: Andrographis paniculata, antioxidant potential, methanol, acetone, water, DPPH, ABTS  
Highlights
The use of herbal medicines has rapidly increased in the last few decades and is expanding swiftly across the world day by day (Ekor, 2014; Welz et al, 2018; Ali et al, 2019)
Crude extracts of A. paniculata have been investigated for methanol, acetone, and water extracts
Antioxidant potential was assessed by the Diphenyl-2-picryl hydrazyl (DPPH) scavenging activity, reducing power assay, total antioxidant capacity, ABTS radical scavenging activity, nitric oxide (NO) scavenging activity, amount of total phenolic and flavonoid content determination
Summary
The use of herbal medicines has rapidly increased in the last few decades and is expanding swiftly across the world day by day (Ekor, 2014; Welz et al, 2018; Ali et al, 2019). Some herbal medicines have been known for their potential biological activity, and are widely used, the vast majority of them yet remain untested, and their uses are poorly monitored as well. This scenario is prevalent in Bangladesh, where herbal medicines play a pivotal role in the treatment of different diseases. In Bangladesh, A. paniculata commonly referred to as ' Kalmegh' in Bangla, has markedly been pervasively consumed for ages as one of the most vital medicinal plants
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