Abstract
Collagen is the main structural component of connective tissues in vertebral and invertebral animal tissues and organs. The collagen isolated from the body wall of sea cucumber (Holothuria lecospilota) can be used in the pharmaceutical field. The research aimed at isolating collagen from the sea cucumber body wall and testing the anti-tyrosinase and anti-elastase activity. This was done by isolation of collagen by soaking the body wall of sea cucumber using Tris-HCl-EDTA 0.1 M and dialyzing using 0.5 M acetic acid. The step was followed by characterization of collagen using FT-IR spectrophotometer and sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). The resulting collagen was tested for anti-tyrosinase and anti-elastase in vitro. The research showed that the resulting collagen was of the amide group with molecular weight of 166.43 and 138.35 kDa, showing α1 and α2 chains. Anti-tyrosinase activity test showed an IC50 value of 1.20 mg/mL, while anti-elastase activity test showed an IC50 value of 125 µg/mL. The collagen isolated from the sea cucumber has weak anti-tyrosinase and moderate anti-elastase activity. Key words: Crude collagen, anti-tyrosinase, anti-elastase, sea cucumber (Holothuria leucospilota).
Highlights
The study aimed at isolating collagen from the body wall of sea cucumber and conduct in vitro test for anti-tyrosinase and anti-elastase activity in sea cucumber
The body wall of H. leucospilota was dissected for free adherent tissues, cut into small pieces, and stored in a small container filled with phosphate buffer saline (PBS)
The dissected body wall of sea cucumber was soaked in 0.1 M NaOH solution for 6 h, soaked in 0.1 M pH 8.0 Tris-HCl for 24 h, washed using pH 7.0 double destilled water, and stirred using 0.5 M acetic acid for 48 and 72 h
Summary
Besides its use for diet purpose, sea cucumber has promising marine-derived medicinal potentials (Bordbar et al, 2001). It can be used for its anti- cancer, bacterial and malarial activities (Janakiram et al, 2005; Layson et al, 2014; Liu et al, 2007). Marine organisms have been recognized as potential alternative sources, due to their availability, lack of dietary restriction, lack of disease risk and high collagen yields (Liu et al, 2007). Few studies have been conducted on sea cucumber from Indonesia, while collagen is widely used in pharmaceutical and cosmetic industries. The study aimed at isolating collagen from the body wall of sea cucumber and conduct in vitro test for anti-tyrosinase and anti-elastase activity in sea cucumber
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